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Direct protein transfer to terminally differentiated muscle cells.

Derer, W and Easwaran, H. P. and Knopf, C. W. and Leonhardt, H. and Cardoso, M Cristina (1999):
Direct protein transfer to terminally differentiated muscle cells.
In: Journal of molecular medicine, 77 (8), pp. 609-13, ISSN 0946-2716,
[Online-Edition: http://www.cardoso-lab.org/publications/Derer_1999.pdf],
[Article]

Abstract

Recently, a new approach for direct protein transfer to mammalian cells based on the herpes simplex virus type 1 protein VP22 has been described. This protein has the remarkable property of intercellular trafficking, which is independent of direct cell contacts and is also retained when fused to heterologous proteins. However, the spreading has only been described for proliferating cells and has also been controversially discussed. In this study we describe the generation of a GFP-VP22 fusion protein which is able to spread in COS-7 cells after transient transfection. Moreover, we show in coculture experiments with transfected COS-7 cells and C2C12 myotubes that this fusion protein is also able to spread into terminally differentiated skeletal muscle cells. These results suggest that VP22 might be a novel therapeutic tool for direct protein transfer not only in proliferating but also in terminally differentiated cells.

Item Type: Article
Erschienen: 1999
Creators: Derer, W and Easwaran, H. P. and Knopf, C. W. and Leonhardt, H. and Cardoso, M Cristina
Title: Direct protein transfer to terminally differentiated muscle cells.
Language: German
Abstract:

Recently, a new approach for direct protein transfer to mammalian cells based on the herpes simplex virus type 1 protein VP22 has been described. This protein has the remarkable property of intercellular trafficking, which is independent of direct cell contacts and is also retained when fused to heterologous proteins. However, the spreading has only been described for proliferating cells and has also been controversially discussed. In this study we describe the generation of a GFP-VP22 fusion protein which is able to spread in COS-7 cells after transient transfection. Moreover, we show in coculture experiments with transfected COS-7 cells and C2C12 myotubes that this fusion protein is also able to spread into terminally differentiated skeletal muscle cells. These results suggest that VP22 might be a novel therapeutic tool for direct protein transfer not only in proliferating but also in terminally differentiated cells.

Journal or Publication Title: Journal of molecular medicine
Volume: 77
Number: 8
Divisions: 10 Department of Biology > Cell Biology and Epigenetics
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10 Department of Biology
Date Deposited: 05 Mar 2010 15:54
Official URL: http://www.cardoso-lab.org/publications/Derer_1999.pdf
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