Derer, W ; Easwaran, H. P. ; Knopf, C. W. ; Leonhardt, H. ; Cardoso, M. Cristina (1999)
Direct protein transfer to terminally differentiated muscle cells.
In: Journal of molecular medicine, 77 (8)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Recently, a new approach for direct protein transfer to mammalian cells based on the herpes simplex virus type 1 protein VP22 has been described. This protein has the remarkable property of intercellular trafficking, which is independent of direct cell contacts and is also retained when fused to heterologous proteins. However, the spreading has only been described for proliferating cells and has also been controversially discussed. In this study we describe the generation of a GFP-VP22 fusion protein which is able to spread in COS-7 cells after transient transfection. Moreover, we show in coculture experiments with transfected COS-7 cells and C2C12 myotubes that this fusion protein is also able to spread into terminally differentiated skeletal muscle cells. These results suggest that VP22 might be a novel therapeutic tool for direct protein transfer not only in proliferating but also in terminally differentiated cells.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1999 |
| Autor(en): | Derer, W ; Easwaran, H. P. ; Knopf, C. W. ; Leonhardt, H. ; Cardoso, M. Cristina |
| Art des Eintrags: | Bibliographie |
| Titel: | Direct protein transfer to terminally differentiated muscle cells. |
| Sprache: | Deutsch |
| Publikationsjahr: | 1999 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of molecular medicine |
| Jahrgang/Volume einer Zeitschrift: | 77 |
| (Heft-)Nummer: | 8 |
| URL / URN: | http://www.cardoso-lab.org/publications/Derer_1999.pdf |
| Kurzbeschreibung (Abstract): | Recently, a new approach for direct protein transfer to mammalian cells based on the herpes simplex virus type 1 protein VP22 has been described. This protein has the remarkable property of intercellular trafficking, which is independent of direct cell contacts and is also retained when fused to heterologous proteins. However, the spreading has only been described for proliferating cells and has also been controversially discussed. In this study we describe the generation of a GFP-VP22 fusion protein which is able to spread in COS-7 cells after transient transfection. Moreover, we show in coculture experiments with transfected COS-7 cells and C2C12 myotubes that this fusion protein is also able to spread into terminally differentiated skeletal muscle cells. These results suggest that VP22 might be a novel therapeutic tool for direct protein transfer not only in proliferating but also in terminally differentiated cells. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Cell Biology and Epigenetics ?? fb10_zoologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 05 Mär 2010 15:54 |
| Letzte Änderung: | 05 Mär 2013 09:32 |
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