Cardoso, M. Cristina ; Leonhardt, H. (1999)
DNA methyltransferase is actively retained in the cytoplasm during early development.
In: The Journal of cell biology, 147 (1)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The overall DNA methylation level sharply decreases from the zygote to the blastocyst stage despite the presence of high levels of DNA methyltransferase (Dnmt1). Surprisingly, the enzyme is localized in the cytoplasm of early embryos despite the presence of several functional nuclear localization signals. We mapped a region in the NH(2)-terminal, regulatory domain of Dnmt1 that is necessary and sufficient for cytoplasmic retention during early development. Altogether, our results suggest that Dnmt1 is actively retained in the cytoplasm, which prevents binding to its DNA substrate in the nucleus and thereby contributes to the erasure of gamete-specific epigenetic information during early mammalian development.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1999 |
| Autor(en): | Cardoso, M. Cristina ; Leonhardt, H. |
| Art des Eintrags: | Bibliographie |
| Titel: | DNA methyltransferase is actively retained in the cytoplasm during early development. |
| Sprache: | Englisch |
| Publikationsjahr: | 1999 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | The Journal of cell biology |
| Jahrgang/Volume einer Zeitschrift: | 147 |
| (Heft-)Nummer: | 1 |
| URL / URN: | http://www.cardoso-lab.org/publications/Cardoso_1999b.pdf |
| Kurzbeschreibung (Abstract): | The overall DNA methylation level sharply decreases from the zygote to the blastocyst stage despite the presence of high levels of DNA methyltransferase (Dnmt1). Surprisingly, the enzyme is localized in the cytoplasm of early embryos despite the presence of several functional nuclear localization signals. We mapped a region in the NH(2)-terminal, regulatory domain of Dnmt1 that is necessary and sufficient for cytoplasmic retention during early development. Altogether, our results suggest that Dnmt1 is actively retained in the cytoplasm, which prevents binding to its DNA substrate in the nucleus and thereby contributes to the erasure of gamete-specific epigenetic information during early mammalian development. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_zoologie ?? 10 Fachbereich Biologie > Cell Biology and Epigenetics |
| Hinterlegungsdatum: | 05 Mär 2010 15:54 |
| Letzte Änderung: | 17 Dez 2018 15:17 |
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