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Mammalian DNA methyltransferases show different subnuclear distributions.

Margot, J. B. ; Cardoso, M. Cristina ; Leonhardt, H. (2001)
Mammalian DNA methyltransferases show different subnuclear distributions.
In: Journal of cellular biochemistry, 83 (3)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

In mammalian cells, DNA methylation patterns are precisely maintained after DNA replication with defined changes occurring during development. The major DNA methyltransferase (Dnmt1) is associated with nuclear replication sites during S-phase, which is consistent with a role in maintenance methylation. The subcellular distribution of the recently discovered de novo DNA methyltransferases, Dnmt3a and Dnmt3b, was investigated by immunofluorescence and by epitope tagging. We now show that both Dnmt3a and Dnmt3b are distributed throughout the nucleoplasm but are not associated with nuclear DNA replication sites during S-phase. These results suggest that de novo methylation by Dnmt3a and Dnmt3b occurs independently of the replication process and might involve an alternative mechanism for accessing the target DNA. The different subcellular distribution of mammalian DNA methyltransferases might thus contribute to the regulation of DNA methylation.

Typ des Eintrags: Artikel
Erschienen: 2001
Autor(en): Margot, J. B. ; Cardoso, M. Cristina ; Leonhardt, H.
Art des Eintrags: Bibliographie
Titel: Mammalian DNA methyltransferases show different subnuclear distributions.
Sprache: Englisch
Publikationsjahr: 2001
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Journal of cellular biochemistry
Jahrgang/Volume einer Zeitschrift: 83
(Heft-)Nummer: 3
URL / URN: http://www.cardoso-lab.org/publications/Margot_2001.pdf
Kurzbeschreibung (Abstract):

In mammalian cells, DNA methylation patterns are precisely maintained after DNA replication with defined changes occurring during development. The major DNA methyltransferase (Dnmt1) is associated with nuclear replication sites during S-phase, which is consistent with a role in maintenance methylation. The subcellular distribution of the recently discovered de novo DNA methyltransferases, Dnmt3a and Dnmt3b, was investigated by immunofluorescence and by epitope tagging. We now show that both Dnmt3a and Dnmt3b are distributed throughout the nucleoplasm but are not associated with nuclear DNA replication sites during S-phase. These results suggest that de novo methylation by Dnmt3a and Dnmt3b occurs independently of the replication process and might involve an alternative mechanism for accessing the target DNA. The different subcellular distribution of mammalian DNA methyltransferases might thus contribute to the regulation of DNA methylation.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
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10 Fachbereich Biologie > Cell Biology and Epigenetics
Hinterlegungsdatum: 06 Mär 2010 07:18
Letzte Änderung: 17 Dez 2018 15:16
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