Margot, J. B. ; Cardoso, M. Cristina ; Leonhardt, H. (2001)
Mammalian DNA methyltransferases show different subnuclear distributions.
In: Journal of cellular biochemistry, 83 (3)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
In mammalian cells, DNA methylation patterns are precisely maintained after DNA replication with defined changes occurring during development. The major DNA methyltransferase (Dnmt1) is associated with nuclear replication sites during S-phase, which is consistent with a role in maintenance methylation. The subcellular distribution of the recently discovered de novo DNA methyltransferases, Dnmt3a and Dnmt3b, was investigated by immunofluorescence and by epitope tagging. We now show that both Dnmt3a and Dnmt3b are distributed throughout the nucleoplasm but are not associated with nuclear DNA replication sites during S-phase. These results suggest that de novo methylation by Dnmt3a and Dnmt3b occurs independently of the replication process and might involve an alternative mechanism for accessing the target DNA. The different subcellular distribution of mammalian DNA methyltransferases might thus contribute to the regulation of DNA methylation.
Typ des Eintrags: | Artikel |
---|---|
Erschienen: | 2001 |
Autor(en): | Margot, J. B. ; Cardoso, M. Cristina ; Leonhardt, H. |
Art des Eintrags: | Bibliographie |
Titel: | Mammalian DNA methyltransferases show different subnuclear distributions. |
Sprache: | Englisch |
Publikationsjahr: | 2001 |
Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of cellular biochemistry |
Jahrgang/Volume einer Zeitschrift: | 83 |
(Heft-)Nummer: | 3 |
URL / URN: | http://www.cardoso-lab.org/publications/Margot_2001.pdf |
Kurzbeschreibung (Abstract): | In mammalian cells, DNA methylation patterns are precisely maintained after DNA replication with defined changes occurring during development. The major DNA methyltransferase (Dnmt1) is associated with nuclear replication sites during S-phase, which is consistent with a role in maintenance methylation. The subcellular distribution of the recently discovered de novo DNA methyltransferases, Dnmt3a and Dnmt3b, was investigated by immunofluorescence and by epitope tagging. We now show that both Dnmt3a and Dnmt3b are distributed throughout the nucleoplasm but are not associated with nuclear DNA replication sites during S-phase. These results suggest that de novo methylation by Dnmt3a and Dnmt3b occurs independently of the replication process and might involve an alternative mechanism for accessing the target DNA. The different subcellular distribution of mammalian DNA methyltransferases might thus contribute to the regulation of DNA methylation. |
Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_zoologie ?? 10 Fachbereich Biologie > Cell Biology and Epigenetics |
Hinterlegungsdatum: | 06 Mär 2010 07:18 |
Letzte Änderung: | 17 Dez 2018 15:16 |
PPN: | |
Export: | |
Suche nach Titel in: | TUfind oder in Google |
![]() |
Frage zum Eintrag |
Optionen (nur für Redakteure)
![]() |
Redaktionelle Details anzeigen |