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Stable chromosomal units determine the spatial and temporal organization of DNA replication.

Sadoni, Nicolas and Cardoso, M. Cristina and Stelzer, Ernst H. K. and Leonhardt, Heinrich and Zink, Daniele (2004):
Stable chromosomal units determine the spatial and temporal organization of DNA replication.
In: Journal of cell science, pp. 5353-65, 117, (Pt 22), ISSN 0021-9533, [Online-Edition: http://www.cardoso-lab.org/publications/Sadoni_2004.pdf],
[Article]

Abstract

DNA replication occurs in mammalian cells at so-called replication foci occupying defined nuclear sites at specific times during S phase. It is an unresolved problem how this specific spatiotemporal organization of replication foci is determined. Another unresolved question remains as to what extent DNA is redistributed during S phase. To investigate these problems, we visualized the replicating DNA and the replication machinery simultaneously in living HeLa cells. Time-lapse analyses revealed that DNA was not redistributed to other nuclear sites during S phase. Furthermore, the results showed that DNA is organized into stable aggregates equivalent to replication foci. These aggregates, which we call sub-chromosomal foci, stably maintained their replication timing from S phase to S phase. During S-phase progression, the replication machinery sequentially proceeded through spatially adjacent sets of sub-chromosomal foci. These findings imply that the specific nuclear substructure of chromosomes and the order of their stable subunits determine the spatiotemporal organization of DNA replication.

Item Type: Article
Erschienen: 2004
Creators: Sadoni, Nicolas and Cardoso, M. Cristina and Stelzer, Ernst H. K. and Leonhardt, Heinrich and Zink, Daniele
Title: Stable chromosomal units determine the spatial and temporal organization of DNA replication.
Language: English
Abstract:

DNA replication occurs in mammalian cells at so-called replication foci occupying defined nuclear sites at specific times during S phase. It is an unresolved problem how this specific spatiotemporal organization of replication foci is determined. Another unresolved question remains as to what extent DNA is redistributed during S phase. To investigate these problems, we visualized the replicating DNA and the replication machinery simultaneously in living HeLa cells. Time-lapse analyses revealed that DNA was not redistributed to other nuclear sites during S phase. Furthermore, the results showed that DNA is organized into stable aggregates equivalent to replication foci. These aggregates, which we call sub-chromosomal foci, stably maintained their replication timing from S phase to S phase. During S-phase progression, the replication machinery sequentially proceeded through spatially adjacent sets of sub-chromosomal foci. These findings imply that the specific nuclear substructure of chromosomes and the order of their stable subunits determine the spatiotemporal organization of DNA replication.

Journal or Publication Title: Journal of cell science
Volume: 117
Number: Pt 22
Divisions: 10 Department of Biology
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10 Department of Biology > Cell Biology and Epigenetics
Date Deposited: 06 Mar 2010 07:38
Official URL: http://www.cardoso-lab.org/publications/Sadoni_2004.pdf
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