Sadoni, Nicolas ; Cardoso, M. Cristina ; Stelzer, Ernst H. K. ; Leonhardt, Heinrich ; Zink, Daniele (2004)
Stable chromosomal units determine the spatial and temporal organization of DNA replication.
In: Journal of cell science, 117 (Pt 22)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
DNA replication occurs in mammalian cells at so-called replication foci occupying defined nuclear sites at specific times during S phase. It is an unresolved problem how this specific spatiotemporal organization of replication foci is determined. Another unresolved question remains as to what extent DNA is redistributed during S phase. To investigate these problems, we visualized the replicating DNA and the replication machinery simultaneously in living HeLa cells. Time-lapse analyses revealed that DNA was not redistributed to other nuclear sites during S phase. Furthermore, the results showed that DNA is organized into stable aggregates equivalent to replication foci. These aggregates, which we call sub-chromosomal foci, stably maintained their replication timing from S phase to S phase. During S-phase progression, the replication machinery sequentially proceeded through spatially adjacent sets of sub-chromosomal foci. These findings imply that the specific nuclear substructure of chromosomes and the order of their stable subunits determine the spatiotemporal organization of DNA replication.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2004 |
| Autor(en): | Sadoni, Nicolas ; Cardoso, M. Cristina ; Stelzer, Ernst H. K. ; Leonhardt, Heinrich ; Zink, Daniele |
| Art des Eintrags: | Bibliographie |
| Titel: | Stable chromosomal units determine the spatial and temporal organization of DNA replication. |
| Sprache: | Englisch |
| Publikationsjahr: | 2004 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of cell science |
| Jahrgang/Volume einer Zeitschrift: | 117 |
| (Heft-)Nummer: | Pt 22 |
| URL / URN: | http://www.cardoso-lab.org/publications/Sadoni_2004.pdf |
| Kurzbeschreibung (Abstract): | DNA replication occurs in mammalian cells at so-called replication foci occupying defined nuclear sites at specific times during S phase. It is an unresolved problem how this specific spatiotemporal organization of replication foci is determined. Another unresolved question remains as to what extent DNA is redistributed during S phase. To investigate these problems, we visualized the replicating DNA and the replication machinery simultaneously in living HeLa cells. Time-lapse analyses revealed that DNA was not redistributed to other nuclear sites during S phase. Furthermore, the results showed that DNA is organized into stable aggregates equivalent to replication foci. These aggregates, which we call sub-chromosomal foci, stably maintained their replication timing from S phase to S phase. During S-phase progression, the replication machinery sequentially proceeded through spatially adjacent sets of sub-chromosomal foci. These findings imply that the specific nuclear substructure of chromosomes and the order of their stable subunits determine the spatiotemporal organization of DNA replication. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_zoologie ?? 10 Fachbereich Biologie > Cell Biology and Epigenetics |
| Hinterlegungsdatum: | 06 Mär 2010 07:38 |
| Letzte Änderung: | 29 Jan 2019 15:28 |
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