Grünwald, D. ; Cardoso, M. Cristina ; Leonhardt, H. ; Buschmann, V. (2005)
Diffusion and binding properties investigated by Fluorescence Correlation Spectroscopy (FCS).
In: Current pharmaceutical biotechnology, 6 (5)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
During the last years, Fluorescence Correlation Spectroscopy (FCS) has proven to be a powerful tool for basic research in many applications. The combination of a minimal detection volume in the femtoliter range coupled with very high sensitivity extends the possibilities to design sensitive homogeneous tests. In this article we illustrate the analysis of binding processes with FCS based on the changes in diffusion characteristics of GFP upon binding to an antibody. Problems induced by highly heterogeneous samples are discussed and differences of GFP binding to a monoclonal and a polyclonal antibody are shown and analyzed. We stress data processing, limitations and useful approximations in FCS methodology. Basic ideas of data acquisition and processing as well as new developments and applications are presented.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2005 |
| Autor(en): | Grünwald, D. ; Cardoso, M. Cristina ; Leonhardt, H. ; Buschmann, V. |
| Art des Eintrags: | Bibliographie |
| Titel: | Diffusion and binding properties investigated by Fluorescence Correlation Spectroscopy (FCS). |
| Sprache: | Englisch |
| Publikationsjahr: | 2005 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Current pharmaceutical biotechnology |
| Jahrgang/Volume einer Zeitschrift: | 6 |
| (Heft-)Nummer: | 5 |
| URL / URN: | http://www.cardoso-lab.org/publications/Grunwald_2005.pdf |
| Kurzbeschreibung (Abstract): | During the last years, Fluorescence Correlation Spectroscopy (FCS) has proven to be a powerful tool for basic research in many applications. The combination of a minimal detection volume in the femtoliter range coupled with very high sensitivity extends the possibilities to design sensitive homogeneous tests. In this article we illustrate the analysis of binding processes with FCS based on the changes in diffusion characteristics of GFP upon binding to an antibody. Problems induced by highly heterogeneous samples are discussed and differences of GFP binding to a monoclonal and a polyclonal antibody are shown and analyzed. We stress data processing, limitations and useful approximations in FCS methodology. Basic ideas of data acquisition and processing as well as new developments and applications are presented. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_zoologie ?? 10 Fachbereich Biologie > Cell Biology and Epigenetics |
| Hinterlegungsdatum: | 06 Mär 2010 07:51 |
| Letzte Änderung: | 18 Jul 2018 13:32 |
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