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Cell cycle markers for live cell analyses.

Easwaran, Hariharan P. and Leonhardt, Heinrich and Cardoso, M Cristina :
Cell cycle markers for live cell analyses.
[Online-Edition: http://www.cardoso-lab.org/publications/Easwaran_2005.pdf]
In: Cell cycle (Georgetown, Tex.), 4 (3) pp. 453-5. ISSN 1551-4005
[Article] , (2005)

Official URL: http://www.cardoso-lab.org/publications/Easwaran_2005.pdf

Abstract

Many cellular processes are regulated by cell cycle dependent changes in protein dynamics and localization. Studying these changes in vivo requires methods to distinguish the different cell cycle stages. Here we demonstrate the use of DNA Ligase I fused to DsRed1 as an in situ marker to identify S phase and the subsequent transition to G2 in live cells. Using this marker, we observed changes in the nuclear distribution of Dnmt1 during cell cycle progression. Based on the different nuclear distribution of DNA Ligase I and Dnmt1 in G2 and G1, we demonstrate that the combination of both proteins allows the direct discrimination of all cell cycle phases using either immunostainings or fusions with fluorescent proteins. These markers are new tools to directly study cell cycle dependent processes in both, fixed and living cells.

Item Type: Article
Erschienen: 2005
Creators: Easwaran, Hariharan P. and Leonhardt, Heinrich and Cardoso, M Cristina
Title: Cell cycle markers for live cell analyses.
Language: German
Abstract:

Many cellular processes are regulated by cell cycle dependent changes in protein dynamics and localization. Studying these changes in vivo requires methods to distinguish the different cell cycle stages. Here we demonstrate the use of DNA Ligase I fused to DsRed1 as an in situ marker to identify S phase and the subsequent transition to G2 in live cells. Using this marker, we observed changes in the nuclear distribution of Dnmt1 during cell cycle progression. Based on the different nuclear distribution of DNA Ligase I and Dnmt1 in G2 and G1, we demonstrate that the combination of both proteins allows the direct discrimination of all cell cycle phases using either immunostainings or fusions with fluorescent proteins. These markers are new tools to directly study cell cycle dependent processes in both, fixed and living cells.

Journal or Publication Title: Cell cycle (Georgetown, Tex.)
Volume: 4
Number: 3
Divisions: 10 Department of Biology > Cell Biology and Epigenetics
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10 Department of Biology
Date Deposited: 06 Mar 2010 07:55
Official URL: http://www.cardoso-lab.org/publications/Easwaran_2005.pdf
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