Rothbauer, Ulrich ; Zolghadr, Kourosh ; Tillib, Sergei ; Nowak, Danny ; Schermelleh, Lothar ; Gahl, Anja ; Backmann, Natalija ; Conrath, Katja ; Muyldermans, Serge ; Cardoso, M. Cristina ; Leonhardt, Heinrich (2006)
Targeting and tracing antigens in live cells with fluorescent nanobodies.
In: Nature methods, 3 (11)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2006 |
| Autor(en): | Rothbauer, Ulrich ; Zolghadr, Kourosh ; Tillib, Sergei ; Nowak, Danny ; Schermelleh, Lothar ; Gahl, Anja ; Backmann, Natalija ; Conrath, Katja ; Muyldermans, Serge ; Cardoso, M. Cristina ; Leonhardt, Heinrich |
| Art des Eintrags: | Bibliographie |
| Titel: | Targeting and tracing antigens in live cells with fluorescent nanobodies. |
| Sprache: | Deutsch |
| Publikationsjahr: | 2006 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Nature methods |
| Jahrgang/Volume einer Zeitschrift: | 3 |
| (Heft-)Nummer: | 11 |
| URL / URN: | http://www.cardoso-lab.org/publications/Rothbauer%20et%20al.... |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Cell Biology and Epigenetics ?? fb10_zoologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 06 Mär 2010 08:16 |
| Letzte Änderung: | 05 Mär 2013 09:32 |
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