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MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation.

Agarwal, Noopur and Hardt, Tanja and Brero, Alessandro and Nowak, Danny and Rothbauer, Ulrich and Becker, Annette and Leonhardt, Heinrich and Cardoso, M Cristina (2007):
MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation.
In: Nucleic acids research, pp. 5402-8, 35, (16), ISSN 1362-4962, [Online-Edition: http://www.cardoso-lab.org/publications/Agarwal%202007.pdf],
[Article]

Abstract

There is increasing evidence of crosstalk between epigenetic modifications such as histone and DNA methylation, recognized by HP1 and methyl CpG-binding proteins, respectively. We have previously shown that the level of methyl CpG-binding proteins increased dramatically during myogenesis leading to large-scale heterochromatin reorganization. In this work, we show that the level of HP1 isoforms did not change significantly throughout myogenic differentiation but their localization did. In particular, HP1gamma relocalization to heterochromatin correlated with MeCP2 presence. Using co-immunoprecipitation assays, we found that these heterochromatic factors interact in vivo via the chromo shadow domain of HP1 and the first 55 amino acids of MeCP2. We propose that this dynamic interaction of HP1 and MeCP2 increases their concentration at heterochromatin linking two major gene silencing pathways to stabilize transcriptional repression during differentiation.

Item Type: Article
Erschienen: 2007
Creators: Agarwal, Noopur and Hardt, Tanja and Brero, Alessandro and Nowak, Danny and Rothbauer, Ulrich and Becker, Annette and Leonhardt, Heinrich and Cardoso, M Cristina
Title: MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation.
Language: English
Abstract:

There is increasing evidence of crosstalk between epigenetic modifications such as histone and DNA methylation, recognized by HP1 and methyl CpG-binding proteins, respectively. We have previously shown that the level of methyl CpG-binding proteins increased dramatically during myogenesis leading to large-scale heterochromatin reorganization. In this work, we show that the level of HP1 isoforms did not change significantly throughout myogenic differentiation but their localization did. In particular, HP1gamma relocalization to heterochromatin correlated with MeCP2 presence. Using co-immunoprecipitation assays, we found that these heterochromatic factors interact in vivo via the chromo shadow domain of HP1 and the first 55 amino acids of MeCP2. We propose that this dynamic interaction of HP1 and MeCP2 increases their concentration at heterochromatin linking two major gene silencing pathways to stabilize transcriptional repression during differentiation.

Journal or Publication Title: Nucleic acids research
Volume: 35
Number: 16
Divisions: 10 Department of Biology > Cell Biology and Epigenetics
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10 Department of Biology
Date Deposited: 06 Mar 2010 15:49
Official URL: http://www.cardoso-lab.org/publications/Agarwal%202007.pdf
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