Agarwal, Noopur ; Hardt, Tanja ; Brero, Alessandro ; Nowak, Danny ; Rothbauer, Ulrich ; Becker, Annette ; Leonhardt, Heinrich ; Cardoso, M. Cristina (2007)
MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation.
In: Nucleic acids research, 35 (16)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
There is increasing evidence of crosstalk between epigenetic modifications such as histone and DNA methylation, recognized by HP1 and methyl CpG-binding proteins, respectively. We have previously shown that the level of methyl CpG-binding proteins increased dramatically during myogenesis leading to large-scale heterochromatin reorganization. In this work, we show that the level of HP1 isoforms did not change significantly throughout myogenic differentiation but their localization did. In particular, HP1gamma relocalization to heterochromatin correlated with MeCP2 presence. Using co-immunoprecipitation assays, we found that these heterochromatic factors interact in vivo via the chromo shadow domain of HP1 and the first 55 amino acids of MeCP2. We propose that this dynamic interaction of HP1 and MeCP2 increases their concentration at heterochromatin linking two major gene silencing pathways to stabilize transcriptional repression during differentiation.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2007 |
| Autor(en): | Agarwal, Noopur ; Hardt, Tanja ; Brero, Alessandro ; Nowak, Danny ; Rothbauer, Ulrich ; Becker, Annette ; Leonhardt, Heinrich ; Cardoso, M. Cristina |
| Art des Eintrags: | Bibliographie |
| Titel: | MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation. |
| Sprache: | Englisch |
| Publikationsjahr: | 2007 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Nucleic acids research |
| Jahrgang/Volume einer Zeitschrift: | 35 |
| (Heft-)Nummer: | 16 |
| URL / URN: | http://www.cardoso-lab.org/publications/Agarwal%202007.pdf |
| Kurzbeschreibung (Abstract): | There is increasing evidence of crosstalk between epigenetic modifications such as histone and DNA methylation, recognized by HP1 and methyl CpG-binding proteins, respectively. We have previously shown that the level of methyl CpG-binding proteins increased dramatically during myogenesis leading to large-scale heterochromatin reorganization. In this work, we show that the level of HP1 isoforms did not change significantly throughout myogenic differentiation but their localization did. In particular, HP1gamma relocalization to heterochromatin correlated with MeCP2 presence. Using co-immunoprecipitation assays, we found that these heterochromatic factors interact in vivo via the chromo shadow domain of HP1 and the first 55 amino acids of MeCP2. We propose that this dynamic interaction of HP1 and MeCP2 increases their concentration at heterochromatin linking two major gene silencing pathways to stabilize transcriptional repression during differentiation. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Cell Biology and Epigenetics ?? fb10_zoologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 06 Mär 2010 15:49 |
| Letzte Änderung: | 05 Mär 2013 09:32 |
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