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Generation and characterization of a rat monoclonal antibody specific for PCNA.

Rottach, Andrea ; Kremmer, Elisabeth ; Nowak, Danny ; Boisguerin, Prisca ; Volkmer, Rudolf ; Cardoso, M. Cristina ; Leonhardt, Heinrich ; Rothbauer, Ulrich (2008)
Generation and characterization of a rat monoclonal antibody specific for PCNA.
In: Hybridoma, 27 (2)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Proliferating cell nuclear antigen (PCNA) is a homotrimeric ring-shaped protein that encircles the DNA and acts as a stationary loading platform for multiple, transiently interacting partners participating in various DNA transactions. This essential cellular component, originally characterized as a nuclear antigen of dividing cells, is evolutionary highly conserved from yeast to human. Within the eukaryotic cell, PCNA serves as a processivity factor for DNA polymerase delta and plays a key role in DNA replication, repair, cell cycle regulation, and post-replicative transactions like DNA methylation and chromatin remodelling. All these cellular processes are regulated by a complex network comprising cell cycle dependent changes in expression levels, dynamics, interactions, and localization of PCNA. Here we report the generation and characterization of the first rat monoclonal antibody (MAb) against human PCNA, designated as PCNA 16D10. We demonstrated that PCNA 16D10 MAb has high affinity and specificity and is suited for ELISA, immunoblotting, immunoprecipitation, and immunofluorescence. The characteristic punctate staining of S phase cells allows the identification of proliferating cells and the monitoring of cell cycle progression.

Typ des Eintrags: Artikel
Erschienen: 2008
Autor(en): Rottach, Andrea ; Kremmer, Elisabeth ; Nowak, Danny ; Boisguerin, Prisca ; Volkmer, Rudolf ; Cardoso, M. Cristina ; Leonhardt, Heinrich ; Rothbauer, Ulrich
Art des Eintrags: Bibliographie
Titel: Generation and characterization of a rat monoclonal antibody specific for PCNA.
Sprache: Englisch
Publikationsjahr: 2008
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Hybridoma
Jahrgang/Volume einer Zeitschrift: 27
(Heft-)Nummer: 2
URL / URN: http://www.cardoso-lab.org/publications/Rottach_2008a.pdf
Kurzbeschreibung (Abstract):

Proliferating cell nuclear antigen (PCNA) is a homotrimeric ring-shaped protein that encircles the DNA and acts as a stationary loading platform for multiple, transiently interacting partners participating in various DNA transactions. This essential cellular component, originally characterized as a nuclear antigen of dividing cells, is evolutionary highly conserved from yeast to human. Within the eukaryotic cell, PCNA serves as a processivity factor for DNA polymerase delta and plays a key role in DNA replication, repair, cell cycle regulation, and post-replicative transactions like DNA methylation and chromatin remodelling. All these cellular processes are regulated by a complex network comprising cell cycle dependent changes in expression levels, dynamics, interactions, and localization of PCNA. Here we report the generation and characterization of the first rat monoclonal antibody (MAb) against human PCNA, designated as PCNA 16D10. We demonstrated that PCNA 16D10 MAb has high affinity and specificity and is suited for ELISA, immunoblotting, immunoprecipitation, and immunofluorescence. The characteristic punctate staining of S phase cells allows the identification of proliferating cells and the monitoring of cell cycle progression.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie > Cell Biology and Epigenetics
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10 Fachbereich Biologie
Hinterlegungsdatum: 06 Mär 2010 15:58
Letzte Änderung: 05 Mär 2013 09:32
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