Nitschke, Matthias ; Korte, Thomas ; Tielesch, Claudia ; Ter-Avetisyan, Gohar ; Tünnemann, Gisela ; Cardoso, M. Cristina ; Veit, Michael ; Herrmann, Andreas (2008)
Equine arteritis virus is delivered to an acidic compartment of host cells via clathrin-dependent endocytosis.
In: Virology, 377 (2)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae. Infection by EAV requires the release of the viral genome by fusion with the respective target membrane of the host cell. We have investigated the entry pathway of EAV into Baby Hamster Kidney cells (BHK). Infection of cells assessed by the plaque reduction assay was strongly inhibited by substances which interfere with clathrin-dependent endocytosis and by lysosomotropic compounds. Furthermore, infection of BHK cells was suppressed when clathrin-dependent endocytosis was inhibited by expression of antisense RNA of the clathrin-heavy chain before infection. These results strongly suggest that EAV is taken up via clathrin-dependent endocytosis and is delivered to acidic endosomal compartments.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2008 |
| Autor(en): | Nitschke, Matthias ; Korte, Thomas ; Tielesch, Claudia ; Ter-Avetisyan, Gohar ; Tünnemann, Gisela ; Cardoso, M. Cristina ; Veit, Michael ; Herrmann, Andreas |
| Art des Eintrags: | Bibliographie |
| Titel: | Equine arteritis virus is delivered to an acidic compartment of host cells via clathrin-dependent endocytosis. |
| Sprache: | Englisch |
| Publikationsjahr: | 2008 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Virology |
| Jahrgang/Volume einer Zeitschrift: | 377 |
| (Heft-)Nummer: | 2 |
| URL / URN: | http://www.cardoso-lab.org/publications/Nitschke_2008.pdf |
| Kurzbeschreibung (Abstract): | Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae. Infection by EAV requires the release of the viral genome by fusion with the respective target membrane of the host cell. We have investigated the entry pathway of EAV into Baby Hamster Kidney cells (BHK). Infection of cells assessed by the plaque reduction assay was strongly inhibited by substances which interfere with clathrin-dependent endocytosis and by lysosomotropic compounds. Furthermore, infection of BHK cells was suppressed when clathrin-dependent endocytosis was inhibited by expression of antisense RNA of the clathrin-heavy chain before infection. These results strongly suggest that EAV is taken up via clathrin-dependent endocytosis and is delivered to acidic endosomal compartments. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Cell Biology and Epigenetics ?? fb10_zoologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 06 Mär 2010 15:59 |
| Letzte Änderung: | 05 Mär 2013 09:32 |
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