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Spatiotemporal dynamics of regulatory protein recruitment at DNA damage sites.

Mortusewicz, Oliver and Leonhardt, Heinrich and Cardoso, M Cristina (2008):
Spatiotemporal dynamics of regulatory protein recruitment at DNA damage sites.
In: Journal of cellular biochemistry, pp. 1562-9, 104, (5), ISSN 1097-4644,
[Online-Edition: http://www.cardoso-lab.org/publications/Mortusewicz_2008.pdf],
[Article]

Abstract

Mammalian cells are constantly threatened by multiple types of DNA lesions arising from various sources like irradiation, environmental agents, replication errors or by-products of the normal cellular metabolism. If not readily detected and repaired these lesions can lead to cell death or to the transformation of cells giving rise to life-threatening diseases like cancer. Multiple specialized repair pathways have evolved to preserve the genetic integrity of a cell. The increasing number of DNA damage sensors, checkpoint regulators, and repair factors identified in the numerous interconnected repair pathways raises the question of how DNA repair is coordinated. In the last decade, various methods have been developed that allow the induction of DNA lesions and subsequent real-time analysis of repair factor assembly at DNA repair sites in living cells. This combination of biophysical and molecular cell biology methods has yielded interesting new insights into the order and kinetics of protein recruitment and identified regulatory sequences and selective loading platforms for the efficient restoration of the genetic and epigenetic integrity of mammalian cells.

Item Type: Article
Erschienen: 2008
Creators: Mortusewicz, Oliver and Leonhardt, Heinrich and Cardoso, M Cristina
Title: Spatiotemporal dynamics of regulatory protein recruitment at DNA damage sites.
Language: English
Abstract:

Mammalian cells are constantly threatened by multiple types of DNA lesions arising from various sources like irradiation, environmental agents, replication errors or by-products of the normal cellular metabolism. If not readily detected and repaired these lesions can lead to cell death or to the transformation of cells giving rise to life-threatening diseases like cancer. Multiple specialized repair pathways have evolved to preserve the genetic integrity of a cell. The increasing number of DNA damage sensors, checkpoint regulators, and repair factors identified in the numerous interconnected repair pathways raises the question of how DNA repair is coordinated. In the last decade, various methods have been developed that allow the induction of DNA lesions and subsequent real-time analysis of repair factor assembly at DNA repair sites in living cells. This combination of biophysical and molecular cell biology methods has yielded interesting new insights into the order and kinetics of protein recruitment and identified regulatory sequences and selective loading platforms for the efficient restoration of the genetic and epigenetic integrity of mammalian cells.

Journal or Publication Title: Journal of cellular biochemistry
Volume: 104
Number: 5
Divisions: 10 Department of Biology > Cell Biology and Epigenetics
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10 Department of Biology
Date Deposited: 06 Mar 2010 16:01
Official URL: http://www.cardoso-lab.org/publications/Mortusewicz_2008.pdf
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