Cremer, Marion ; Brandstetter, Katharina ; Maiser, Andreas ; Rao, Suhas S. P. ; Schmid, Volker J. ; Guirao-Ortiz, Miguel ; Mitra, Namita ; Mamberti, Stefania ; Klein, Kyle N. ; Gilbert, David M. ; Leonhardt, Heinrich ; Cardoso, M. Cristina ; Lieberman Aiden, Erez ; Harz, Hartmann ; Cremer, Thomas (2020)
Cohesin depleted cells rebuild functional nuclear compartments after endomitosis.
In: Nature Communications, 11 (1)
doi: 10.1038/s41467-020-19876-6
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
Cohesin plays an essential role in chromatin loop extrusion, but its impact on a compartmentalized nuclear architecture, linked to nuclear functions, is less well understood. Using live-cell and super-resolved 3D microscopy, here we find that cohesin depletion in a human colon cancer derived cell line results in endomitosis and a single multilobulated nucleus with chromosome territories pervaded by interchromatin channels. Chromosome territories contain chromatin domain clusters with a zonal organization of repressed chromatin domains in the interior and transcriptionally competent domains located at the periphery. These clusters form microscopically defined, active and inactive compartments, which likely correspond to A/B compartments, which are detected with ensemble Hi-C. Splicing speckles are observed nearby within the lining channel system. We further observe that the multilobulated nuclei, despite continuous absence of cohesin, pass through S-phase with typical spatio-temporal patterns of replication domains. Evidence for structural changes of these domains compared to controls suggests that cohesin is required for their full integrity.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2020 |
| Autor(en): | Cremer, Marion ; Brandstetter, Katharina ; Maiser, Andreas ; Rao, Suhas S. P. ; Schmid, Volker J. ; Guirao-Ortiz, Miguel ; Mitra, Namita ; Mamberti, Stefania ; Klein, Kyle N. ; Gilbert, David M. ; Leonhardt, Heinrich ; Cardoso, M. Cristina ; Lieberman Aiden, Erez ; Harz, Hartmann ; Cremer, Thomas |
| Art des Eintrags: | Bibliographie |
| Titel: | Cohesin depleted cells rebuild functional nuclear compartments after endomitosis |
| Sprache: | Englisch |
| Publikationsjahr: | 1 Dezember 2020 |
| Ort: | London |
| Verlag: | Springer Nature |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Nature Communications |
| Jahrgang/Volume einer Zeitschrift: | 11 |
| (Heft-)Nummer: | 1 |
| Kollation: | 16 Seiten |
| DOI: | 10.1038/s41467-020-19876-6 |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | Cohesin plays an essential role in chromatin loop extrusion, but its impact on a compartmentalized nuclear architecture, linked to nuclear functions, is less well understood. Using live-cell and super-resolved 3D microscopy, here we find that cohesin depletion in a human colon cancer derived cell line results in endomitosis and a single multilobulated nucleus with chromosome territories pervaded by interchromatin channels. Chromosome territories contain chromatin domain clusters with a zonal organization of repressed chromatin domains in the interior and transcriptionally competent domains located at the periphery. These clusters form microscopically defined, active and inactive compartments, which likely correspond to A/B compartments, which are detected with ensemble Hi-C. Splicing speckles are observed nearby within the lining channel system. We further observe that the multilobulated nuclei, despite continuous absence of cohesin, pass through S-phase with typical spatio-temporal patterns of replication domains. Evidence for structural changes of these domains compared to controls suggests that cohesin is required for their full integrity. |
| Freie Schlagworte: | Chromatin structure, DNA replication, Genome, Nuclear organization, Super-resolution microscopy |
| ID-Nummer: | Artikel-ID: 6146 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Cell Biology and Epigenetics |
| Hinterlegungsdatum: | 26 Sep 2024 07:32 |
| Letzte Änderung: | 26 Sep 2024 07:32 |
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| Suche nach Titel in: | TUfind oder in Google |
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Cohesin depleted cells rebuild functional nuclear compartments after endomitosis. (deposited 25 Sep 2024 11:43)
- Cohesin depleted cells rebuild functional nuclear compartments after endomitosis. (deposited 26 Sep 2024 07:32) [Gegenwärtig angezeigt]
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