Ensinger, Wolfgang ; Ali, Mubarak ; Nasir, Saima ; Duznovic, Ivana ; Trautmann, Christina ; Toimil-Molares, Maria Eugenia ; Distefano, Giuseppa R. ; Laube, Bodo ; Bernhard, Max ; MikoschWersching, Melanie ; Schlaak, Helmut F. ; El Khoury, Mario (2018)
The iNAPO Project: biomimetic nanopores for a new generation of lab-on-chip micro sensors.
In: International Journal of Theoretical and Applied Nanotechnology
doi: 10.11159/ijtan.2018.004
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
In nature, ion conducting nanopores play a vital role for the function of living cells. They undergo gating processes where they open and close upon an external stimulus, such as the presence of a particular biomolecule, the ligand. When the gating process is observed and is quantitatively measured, one can derive data about the presence and the amount of the ligand. Hence, the nanopores can be utilized for specific sensing. However, biological nanopores are embedded in a biological cell membrane that is fragile and unstable with respect to storage and application. The iNAPO (ion conducting nanopores) project aims at combining robust polymer-based nanopores with protein-based biological nanopores, thus combining the selectivity and sensitivity of the latter with the stability and processibility of the first ones. This paper describes the different steps in the fabrication of ion conducting nanopores. It begins with ion irradiation of polymer foils, combined with chemical etching of the ion damage tracks into nanopores. By means of chemical coupling reactions, the nanopore walls are functionalized with particular molecules which react or bioconjugate with the molecules to be analyzed. As an example, a recent result on sensing a physiologically active phosphorus-based anion is shown. By means of a complexation reaction with Zn-di(picolyl)amine, the selective measurement of the concentration of the anion pyrophosphate is demonstrated. In the final step of the project, the nanopores will be incorporated into a Lab-on-Chip system for applications in e.g. medical diagnostics and environmental analysis.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2018 |
| Autor(en): | Ensinger, Wolfgang ; Ali, Mubarak ; Nasir, Saima ; Duznovic, Ivana ; Trautmann, Christina ; Toimil-Molares, Maria Eugenia ; Distefano, Giuseppa R. ; Laube, Bodo ; Bernhard, Max ; MikoschWersching, Melanie ; Schlaak, Helmut F. ; El Khoury, Mario |
| Art des Eintrags: | Bibliographie |
| Titel: | The iNAPO Project: biomimetic nanopores for a new generation of lab-on-chip micro sensors |
| Sprache: | Englisch |
| Publikationsjahr: | 31 Mai 2018 |
| Verlag: | Avestia Publishing |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | International Journal of Theoretical and Applied Nanotechnology |
| DOI: | 10.11159/ijtan.2018.004 |
| Kurzbeschreibung (Abstract): | In nature, ion conducting nanopores play a vital role for the function of living cells. They undergo gating processes where they open and close upon an external stimulus, such as the presence of a particular biomolecule, the ligand. When the gating process is observed and is quantitatively measured, one can derive data about the presence and the amount of the ligand. Hence, the nanopores can be utilized for specific sensing. However, biological nanopores are embedded in a biological cell membrane that is fragile and unstable with respect to storage and application. The iNAPO (ion conducting nanopores) project aims at combining robust polymer-based nanopores with protein-based biological nanopores, thus combining the selectivity and sensitivity of the latter with the stability and processibility of the first ones. This paper describes the different steps in the fabrication of ion conducting nanopores. It begins with ion irradiation of polymer foils, combined with chemical etching of the ion damage tracks into nanopores. By means of chemical coupling reactions, the nanopore walls are functionalized with particular molecules which react or bioconjugate with the molecules to be analyzed. As an example, a recent result on sensing a physiologically active phosphorus-based anion is shown. By means of a complexation reaction with Zn-di(picolyl)amine, the selective measurement of the concentration of the anion pyrophosphate is demonstrated. In the final step of the project, the nanopores will be incorporated into a Lab-on-Chip system for applications in e.g. medical diagnostics and environmental analysis. |
| Freie Schlagworte: | ion track etching, polymer membrane, nanopores, biomimetic sensor, nanosensor, current potential measurements |
| Fachbereich(e)/-gebiet(e): | 11 Fachbereich Material- und Geowissenschaften 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft > Fachgebiet Materialanalytik 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft > Fachgebiet Ionenstrahlmodifizierte Materialien |
| Hinterlegungsdatum: | 29 Feb 2024 11:28 |
| Letzte Änderung: | 04 Mär 2024 07:01 |
| PPN: | |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
 |
Frage zum Eintrag |
Optionen (nur für Redakteure)
 |
Redaktionelle Details anzeigen |
Drucken
Impressum