Layer, Paul G. (2024)
In a century from agitated cells to human organoids.
In: Journal of neuroscience methods, 405
doi: 10.1016/j.jneumeth.2024.110083
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Reaching back more than a century, suspension cultures have provided major insights into processes of histogenesis; e.g., cell communication, distinction of self/nonself, cell sorting and cell adhesion. Besides studies on lower animals, the vertebrate retina served as excellent reaggregate model to analyze 3D reconstruction of a complex neural laminar tissue. Methodologically, keeping cells under suspension is essential to achieve tissue organisation in vitro; thereby, the environmental conditions direct the emergent histotypic particulars. Recent progress in regenerative medicine is based to a large extent on human induced pluripotent stem cells (hiPSCs), which are cultured under suspension. Following their genetically directed differentiation into various histologic 3D structures, organoids provide excellent multipurpose in vitro assay models, as well as tissues for repair transplantations. Historically, a nearly fully laminated retinal spheroid from avian embryos was achieved already in 1984, foreshadowing the potential of culturing stem cells under suspension for tissue reconstruction purposes.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2024 |
| Autor(en): | Layer, Paul G. |
| Art des Eintrags: | Bibliographie |
| Titel: | In a century from agitated cells to human organoids |
| Sprache: | Englisch |
| Publikationsjahr: | 20 Februar 2024 |
| Verlag: | Elsevier |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of neuroscience methods |
| Jahrgang/Volume einer Zeitschrift: | 405 |
| DOI: | 10.1016/j.jneumeth.2024.110083 |
| Kurzbeschreibung (Abstract): | Reaching back more than a century, suspension cultures have provided major insights into processes of histogenesis; e.g., cell communication, distinction of self/nonself, cell sorting and cell adhesion. Besides studies on lower animals, the vertebrate retina served as excellent reaggregate model to analyze 3D reconstruction of a complex neural laminar tissue. Methodologically, keeping cells under suspension is essential to achieve tissue organisation in vitro; thereby, the environmental conditions direct the emergent histotypic particulars. Recent progress in regenerative medicine is based to a large extent on human induced pluripotent stem cells (hiPSCs), which are cultured under suspension. Following their genetically directed differentiation into various histologic 3D structures, organoids provide excellent multipurpose in vitro assay models, as well as tissues for repair transplantations. Historically, a nearly fully laminated retinal spheroid from avian embryos was achieved already in 1984, foreshadowing the potential of culturing stem cells under suspension for tissue reconstruction purposes. |
| ID-Nummer: | pmid:38387805 |
| Zusätzliche Informationen: | Artikel-ID: 110083 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Developmental Biology and Neurogenetics |
| Hinterlegungsdatum: | 27 Feb 2024 07:22 |
| Letzte Änderung: | 08 Apr 2024 11:44 |
| PPN: | 515830062 |
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