Nasir, Saima ; Ali, Mubarak ; Ahmed, Ishtiaq ; Niemeyer, Christof M. ; Ensinger, Wolfgang (2020)
Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates.
In: ChemPlusChem, 2020, 85 (3)
doi: 10.25534/tuprints-00011604
Artikel, Zweitveröffentlichung, Verlagsversion
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Kurzbeschreibung (Abstract)
We report a nanofluidic device for the label-free detection of phosphoprotein (PPn) analytes. To achieve this goal, a metal ion chelator, namely 4-[bis(2-pyridylmethyl)aminomethyl]aniline (DPA-NH2) compound was synthesized. Single asymmetric nanofluidic channels were fabricated in polyethylene terephthalate (PET) membranes. The chelator (DPA-NH2) molecules are subsequently immobilized on the nanochannel surface, followed by the zinc ion complexation to afford DPA-Zn2+ chelates, which act as ligand moieties for the specific binding of phosphoproteins. The success of the chemical reaction and biomolecular recognition process that occur in a confined geometry can be monitored from the changes in electrical readout of the nanochannel. The nanofluidic sensor has the ability to sensitively and specifically detect lower concentrations (≥1 nM) of phosphoprotein (albumin and α-casein) in the surrounding environment as evidenced from the significant decrease in ion current flowing through the nanochannels. However, dephosphoproteins such as lysozyme and dephospho- α-casein even at higher concentration (>1 μM) could not induce any significant change in the transmembrane ion flux. This observation indicated the sensitivity and specificity of the proposed nanofluidic sensor towards PPn proteins, and has potential for use in differentiating between phosphoproteins and dephosphoproteins.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2020 |
| Autor(en): | Nasir, Saima ; Ali, Mubarak ; Ahmed, Ishtiaq ; Niemeyer, Christof M. ; Ensinger, Wolfgang |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates |
| Sprache: | Englisch |
| Publikationsjahr: | 2020 |
| Ort: | Darmstadt |
| Publikationsdatum der Erstveröffentlichung: | 2020 |
| Verlag: | Wiley-VCH |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | ChemPlusChem |
| Jahrgang/Volume einer Zeitschrift: | 85 |
| (Heft-)Nummer: | 3 |
| DOI: | 10.25534/tuprints-00011604 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/11604 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichung aus DEAL-Vertrag mit Wiley |
| Kurzbeschreibung (Abstract): | We report a nanofluidic device for the label-free detection of phosphoprotein (PPn) analytes. To achieve this goal, a metal ion chelator, namely 4-[bis(2-pyridylmethyl)aminomethyl]aniline (DPA-NH2) compound was synthesized. Single asymmetric nanofluidic channels were fabricated in polyethylene terephthalate (PET) membranes. The chelator (DPA-NH2) molecules are subsequently immobilized on the nanochannel surface, followed by the zinc ion complexation to afford DPA-Zn2+ chelates, which act as ligand moieties for the specific binding of phosphoproteins. The success of the chemical reaction and biomolecular recognition process that occur in a confined geometry can be monitored from the changes in electrical readout of the nanochannel. The nanofluidic sensor has the ability to sensitively and specifically detect lower concentrations (≥1 nM) of phosphoprotein (albumin and α-casein) in the surrounding environment as evidenced from the significant decrease in ion current flowing through the nanochannels. However, dephosphoproteins such as lysozyme and dephospho- α-casein even at higher concentration (>1 μM) could not induce any significant change in the transmembrane ion flux. This observation indicated the sensitivity and specificity of the proposed nanofluidic sensor towards PPn proteins, and has potential for use in differentiating between phosphoproteins and dephosphoproteins. |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-116041 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 600 Technik, Medizin, angewandte Wissenschaften > 600 Technik |
| Fachbereich(e)/-gebiet(e): | 11 Fachbereich Material- und Geowissenschaften 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft > Fachgebiet Materialanalytik |
| Hinterlegungsdatum: | 05 Apr 2020 19:57 |
| Letzte Änderung: | 09 Aug 2024 06:53 |
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- Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates. (deposited 05 Apr 2020 19:57) [Gegenwärtig angezeigt]
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