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Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates

Nasir, Saima ; Ali, Mubarak ; Ahmed, Ishtiaq ; Niemeyer, Christof M. ; Ensinger, Wolfgang (2020)
Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates.
In: ChemPlusChem, 85 (3)
doi: 10.1002/cplu.202000045
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

We report a nanofluidic device for the label-free detection of phosphoprotein (PPn) analytes. To achieve this goal, a metal ion chelator, namely 4-[bis(2-pyridylmethyl)aminomethyl]aniline (DPA-NH2) compound was synthesized. Single asymmetric nanofluidic channels were fabricated in polyethylene terephthalate (PET) membranes. The chelator (DPA-NH2) molecules are subsequently immobilized on the nanochannel surface, followed by the zinc ion complexation to afford DPA-Zn2+ chelates, which act as ligand moieties for the specific binding of phosphoproteins. The success of the chemical reaction and biomolecular recognition process that occur in a confined geometry can be monitored from the changes in electrical readout of the nanochannel. The nanofluidic sensor has the ability to sensitively and specifically detect lower concentrations (>= 1 nM) of phosphoprotein (albumin and alpha-casein) in the surrounding environment as evidenced from the significant decrease in ion current flowing through the nanochannels. However, dephosphoproteins such as lysozyme and dephospho-alpha-casein even at higher concentration (>1 mu M) could not induce any significant change in the transmembrane ion flux. This observation indicated the sensitivity and specificity of the proposed nanofluidic sensor towards PPn proteins, and has potential for use in differentiating between phosphoproteins and dephosphoproteins.

Typ des Eintrags: Artikel
Erschienen: 2020
Autor(en): Nasir, Saima ; Ali, Mubarak ; Ahmed, Ishtiaq ; Niemeyer, Christof M. ; Ensinger, Wolfgang
Art des Eintrags: Bibliographie
Titel: Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates
Sprache: Englisch
Publikationsjahr: März 2020
Verlag: Wiley-VCH
Titel der Zeitschrift, Zeitung oder Schriftenreihe: ChemPlusChem
Jahrgang/Volume einer Zeitschrift: 85
(Heft-)Nummer: 3
DOI: 10.1002/cplu.202000045
URL / URN: https://doi.org/10.1002/cplu.202000045
Kurzbeschreibung (Abstract):

We report a nanofluidic device for the label-free detection of phosphoprotein (PPn) analytes. To achieve this goal, a metal ion chelator, namely 4-[bis(2-pyridylmethyl)aminomethyl]aniline (DPA-NH2) compound was synthesized. Single asymmetric nanofluidic channels were fabricated in polyethylene terephthalate (PET) membranes. The chelator (DPA-NH2) molecules are subsequently immobilized on the nanochannel surface, followed by the zinc ion complexation to afford DPA-Zn2+ chelates, which act as ligand moieties for the specific binding of phosphoproteins. The success of the chemical reaction and biomolecular recognition process that occur in a confined geometry can be monitored from the changes in electrical readout of the nanochannel. The nanofluidic sensor has the ability to sensitively and specifically detect lower concentrations (>= 1 nM) of phosphoprotein (albumin and alpha-casein) in the surrounding environment as evidenced from the significant decrease in ion current flowing through the nanochannels. However, dephosphoproteins such as lysozyme and dephospho-alpha-casein even at higher concentration (>1 mu M) could not induce any significant change in the transmembrane ion flux. This observation indicated the sensitivity and specificity of the proposed nanofluidic sensor towards PPn proteins, and has potential for use in differentiating between phosphoproteins and dephosphoproteins.

Freie Schlagworte: iomolecules, ion current rectification, ligand-receptor interactions, sensors, synthetic nanochannels
Fachbereich(e)/-gebiet(e): 11 Fachbereich Material- und Geowissenschaften
11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft
11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft > Fachgebiet Materialanalytik
Hinterlegungsdatum: 16 Nov 2020 06:21
Letzte Änderung: 16 Nov 2020 06:21
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