Heinz, Kathrin S. ; Cardoso, M. Cristina (2019)
Targeted Manipulation/Repositioning of Subcellular Structures and Molecules.
In: Methods in molecular biology (Clifton, N.J.), 2038
doi: 10.1007/978-1-4939-9674-2_13
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Technical advances in live-cell imaging have made cell biology into a highly dynamic field, allowing the visualization and quantification of complex processes in individual cells and in real time. To follow changes and to specifically manipulate factors potentially involved in processes like DNA replication, transcription or repair, we set up a universal targeting approach, allowing directed manipulation of subcellular structures and molecules therein. This strategy is based on the very strong and specific interaction of GFP and GFP-binding nanobody. We describe in detail how to set up the targeting approach with appropriate controls, as well as how to improve and validate its efficiency and finally provide exemplary applications.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2019 |
| Autor(en): | Heinz, Kathrin S. ; Cardoso, M. Cristina |
| Art des Eintrags: | Bibliographie |
| Titel: | Targeted Manipulation/Repositioning of Subcellular Structures and Molecules. |
| Sprache: | Englisch |
| Publikationsjahr: | 13 August 2019 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Methods in molecular biology (Clifton, N.J.) |
| Jahrgang/Volume einer Zeitschrift: | 2038 |
| DOI: | 10.1007/978-1-4939-9674-2_13 |
| Kurzbeschreibung (Abstract): | Technical advances in live-cell imaging have made cell biology into a highly dynamic field, allowing the visualization and quantification of complex processes in individual cells and in real time. To follow changes and to specifically manipulate factors potentially involved in processes like DNA replication, transcription or repair, we set up a universal targeting approach, allowing directed manipulation of subcellular structures and molecules therein. This strategy is based on the very strong and specific interaction of GFP and GFP-binding nanobody. We describe in detail how to set up the targeting approach with appropriate controls, as well as how to improve and validate its efficiency and finally provide exemplary applications. |
| ID-Nummer: | pmid:31407286 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Cell Biology and Epigenetics |
| Hinterlegungsdatum: | 26 Aug 2019 09:30 |
| Letzte Änderung: | 26 Aug 2019 09:31 |
| PPN: | |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
 |
Frage zum Eintrag |
Optionen (nur für Redakteure)
 |
Redaktionelle Details anzeigen |
Drucken
Impressum