Hilberg, Valentina ; Avrutina, Olga ; Ebenig, Aileen ; Yanakieva, Desislava ; Meckel, Tobias ; Biesalski, Markus ; Kolmar, Harald (2019)
Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers.
In: Chemistry (Weinheim an der Bergstrasse, Germany), 25 (7)
doi: 10.1002/chem.201804889
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Efficient and reliable methods for the generation of bioactive papers are of growing interest in view of point-of-care testing devices that do not require extensive analytical equipment. Herein, we report on immobilization of functional proteins onto paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on the combination of highly efficient spatially controllable photo-triggered cycloaddition followed by site-specific sortase A-catalyzed transamidation. This site-directed and regiospecific method allowed unidirectional and covalent immobilization of several proteins possessing different functional properties with ramifications for application in paper-based diagnostics.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2019 |
| Autor(en): | Hilberg, Valentina ; Avrutina, Olga ; Ebenig, Aileen ; Yanakieva, Desislava ; Meckel, Tobias ; Biesalski, Markus ; Kolmar, Harald |
| Art des Eintrags: | Bibliographie |
| Titel: | Light-controlled chemoenzymatic immobilization of proteins towards engineering of bioactive papers. |
| Sprache: | Englisch |
| Publikationsjahr: | 1 Februar 2019 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Chemistry (Weinheim an der Bergstrasse, Germany) |
| Jahrgang/Volume einer Zeitschrift: | 25 |
| (Heft-)Nummer: | 7 |
| DOI: | 10.1002/chem.201804889 |
| URL / URN: | https://doi.org/10.1002/chem.201804889 |
| Kurzbeschreibung (Abstract): | Efficient and reliable methods for the generation of bioactive papers are of growing interest in view of point-of-care testing devices that do not require extensive analytical equipment. Herein, we report on immobilization of functional proteins onto paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on the combination of highly efficient spatially controllable photo-triggered cycloaddition followed by site-specific sortase A-catalyzed transamidation. This site-directed and regiospecific method allowed unidirectional and covalent immobilization of several proteins possessing different functional properties with ramifications for application in paper-based diagnostics. |
| ID-Nummer: | pmid:30395364 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Membrane Dynamics |
| Hinterlegungsdatum: | 13 Nov 2018 07:50 |
| Letzte Änderung: | 11 Feb 2019 13:27 |
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