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How to resolve microsecond current fluctuations in single ion channels: The power of beta distributions

Schroeder, Indra (2015)
How to resolve microsecond current fluctuations in single ion channels: The power of beta distributions.
In: Channels, 9 (5)
doi: 10.1080/19336950.2015.1083660
Article, Bibliographie

Abstract

A main ingredient for the understanding of structure/function correlates of ion channels is the quantitative description of single-channel gating and conductance. However, a wealth of information provided from fast current fluctuations beyond the temporal resolution of the recording system is often ignored, even though it is close to the time window accessible to molecular dynamics simulations. This kind of current fluctuations provide a special technical challenge, because individual opening/closing or blocking/unblocking events cannot be resolved, and the resulting averaging over undetected events decreases the single-channel current. Here, I briefly summarize the history of fast-current fluctuation analysis and focus on the so-called “beta distributions.” This tool exploits characteristics of current fluctuation-induced excess noise on the current amplitude histograms to reconstruct the true single-channel current and kinetic parameters. A guideline for the analysis and recent applications demonstrate that a construction of theoretical beta distributions by Markov Model simulations offers maximum flexibility as compared to analytical solutions.

Item Type: Article
Erschienen: 2015
Creators: Schroeder, Indra
Type of entry: Bibliographie
Title: How to resolve microsecond current fluctuations in single ion channels: The power of beta distributions
Language: English
Date: 2015
Publisher: Taylor & Francis
Journal or Publication Title: Channels
Volume of the journal: 9
Issue Number: 5
DOI: 10.1080/19336950.2015.1083660
URL / URN: https://doi.org/10.1080/19336950.2015.1083660
Abstract:

A main ingredient for the understanding of structure/function correlates of ion channels is the quantitative description of single-channel gating and conductance. However, a wealth of information provided from fast current fluctuations beyond the temporal resolution of the recording system is often ignored, even though it is close to the time window accessible to molecular dynamics simulations. This kind of current fluctuations provide a special technical challenge, because individual opening/closing or blocking/unblocking events cannot be resolved, and the resulting averaging over undetected events decreases the single-channel current. Here, I briefly summarize the history of fast-current fluctuation analysis and focus on the so-called “beta distributions.” This tool exploits characteristics of current fluctuation-induced excess noise on the current amplitude histograms to reconstruct the true single-channel current and kinetic parameters. A guideline for the analysis and recent applications demonstrate that a construction of theoretical beta distributions by Markov Model simulations offers maximum flexibility as compared to analytical solutions.

Divisions: 10 Department of Biology > Plant Membrane Biophyscis (20.12.23 renamed in Biology of Algae and Protozoa)
10 Department of Biology
Date Deposited: 23 Feb 2018 07:55
Last Modified: 23 Feb 2018 07:55
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