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Cotranslational intersection between the SRP and GET targeting pathways to the ER of Saccharomyces cerevisiae.

Zhang, Ying ; Schäffer, Thea ; Wölfle, Tina ; Fitzke, Edith ; Thiel, Gerhard ; Rospert, Sabine (2016)
Cotranslational intersection between the SRP and GET targeting pathways to the ER of Saccharomyces cerevisiae.
In: Molecular and cellular biology, 36 (18)
Article, Bibliographie

Abstract

Targeting of transmembrane proteins to the endoplasmic reticulum proceeds via either the SRP or the GET pathway, consisting of Get1-5 and Sgt2. While SRP cotranslationally targets membrane proteins containing one or multiple transmembrane domains, the GET pathway posttranslationally targets proteins containing a single, C-terminal transmembrane domain termed tail-anchor. Here we dissect the role of the SRP and GET pathway in the sorting of homologous, two-membrane spanning K(+) channel proteins termed Kcv, Kesv, and Kesv-VV. We show that Kcv was targeted to the ER cotranslationally via its N-terminal transmembrane domain, while Kesv-VV was targeted posttranslationally via its C-terminal transmembrane domain, which recruited Get4-5/Sgt2 and Get3. Unexpectedly, nascent Kcv did not only recruit SRP, but also the Get4-5 module of the GET pathway to ribosomes. Ribosome-binding of Get4-5 was independent of Sgt2 and was strongly outcompeted by SRP. The combined data indicate a previously unrecognized cotranslational interplay between the SRP and GET pathways.

Item Type: Article
Erschienen: 2016
Creators: Zhang, Ying ; Schäffer, Thea ; Wölfle, Tina ; Fitzke, Edith ; Thiel, Gerhard ; Rospert, Sabine
Type of entry: Bibliographie
Title: Cotranslational intersection between the SRP and GET targeting pathways to the ER of Saccharomyces cerevisiae.
Language: English
Date: 2016
Journal or Publication Title: Molecular and cellular biology
Volume of the journal: 36
Issue Number: 18
Abstract:

Targeting of transmembrane proteins to the endoplasmic reticulum proceeds via either the SRP or the GET pathway, consisting of Get1-5 and Sgt2. While SRP cotranslationally targets membrane proteins containing one or multiple transmembrane domains, the GET pathway posttranslationally targets proteins containing a single, C-terminal transmembrane domain termed tail-anchor. Here we dissect the role of the SRP and GET pathway in the sorting of homologous, two-membrane spanning K(+) channel proteins termed Kcv, Kesv, and Kesv-VV. We show that Kcv was targeted to the ER cotranslationally via its N-terminal transmembrane domain, while Kesv-VV was targeted posttranslationally via its C-terminal transmembrane domain, which recruited Get4-5/Sgt2 and Get3. Unexpectedly, nascent Kcv did not only recruit SRP, but also the Get4-5 module of the GET pathway to ribosomes. Ribosome-binding of Get4-5 was independent of Sgt2 and was strongly outcompeted by SRP. The combined data indicate a previously unrecognized cotranslational interplay between the SRP and GET pathways.

Divisions: 10 Department of Biology
10 Department of Biology > Plant Membrane Biophyscis (20.12.23 renamed in Biology of Algae and Protozoa)
Date Deposited: 05 Jul 2016 08:40
Last Modified: 04 Oct 2016 07:39
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