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Identification of metE as a Second Target of the sRNA scr5239 in Streptomyces coelicolor.

Vockenhuber, Michael-Paul ; Heueis, Nona ; Suess, Beatrix (2015):
Identification of metE as a Second Target of the sRNA scr5239 in Streptomyces coelicolor.
In: PloS one, 10 (3), pp. e0120147. ISSN 1932-6203,
[Article]

Abstract

While transcriptional regulation of the primary and secondary metabolism of the model organism Streptomyces coelicolor is well studied, little is still known about the role small noncoding RNAs (sRNAs) play in regulating gene expression in this organism. Here, we report the identification of a second target of the sRNA scr5239, an sRNA highly conserved in streptomycetes. The 159 nt long sRNA binds its target, the mRNA of the cobalamin independent methionine synthase metE (SCO0985), at the 5' end of its open reading frame thereby repressing translation. We show that a high methionine level induces expression of scr5239 itself. This leads, in a negative feedback loop, to the repression of methionine biosynthesis. In contrast to the first reported target of this sRNA, the agarase dagA, this interaction seems to be conserved in a wide number of streptomycetes.

Item Type: Article
Erschienen: 2015
Creators: Vockenhuber, Michael-Paul ; Heueis, Nona ; Suess, Beatrix
Title: Identification of metE as a Second Target of the sRNA scr5239 in Streptomyces coelicolor.
Language: English
Abstract:

While transcriptional regulation of the primary and secondary metabolism of the model organism Streptomyces coelicolor is well studied, little is still known about the role small noncoding RNAs (sRNAs) play in regulating gene expression in this organism. Here, we report the identification of a second target of the sRNA scr5239, an sRNA highly conserved in streptomycetes. The 159 nt long sRNA binds its target, the mRNA of the cobalamin independent methionine synthase metE (SCO0985), at the 5' end of its open reading frame thereby repressing translation. We show that a high methionine level induces expression of scr5239 itself. This leads, in a negative feedback loop, to the repression of methionine biosynthesis. In contrast to the first reported target of this sRNA, the agarase dagA, this interaction seems to be conserved in a wide number of streptomycetes.

Journal or Publication Title: PloS one
Journal volume: 10
Number: 3
Divisions: 10 Department of Biology
10 Department of Biology > Synthetic Genetic Circuits
Date Deposited: 30 Mar 2015 13:02
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