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Blood platelet adhesion to printed von Willebrand factor

Davydovskaya, Polina ; Janko, Marek ; Gaertner, Florian ; Ahmad, Zerkah ; Simsek, Özlem ; Maßberg, Steffen ; Stark, Robert W. (2012)
Blood platelet adhesion to printed von Willebrand factor.
In: Journal of Biomedical Materials Research Part A, 100 (2)
doi: 10.1002/jbm.a.33275
Article, Bibliographie

Abstract

Von Willebrand factor (vWF), a glycoprotein in blood, mediates the adhesion of blood platelets and thus plays a crucial role in hemostasis and thrombosis. Functional coating of surfaces with vWF allows the investigation of in vitro adhesion of blood platelet. We used soft lithography to create a functional patterned substrate. vWF was printed on plasma-treated glass and mica surfaces, producing elongated network-like fibril structures. A minimum layer thickness of 3 nm was observed, corresponding to the height of a monolayer of vWF. The stability of the patterns was verified in a laminar fluid flow, and the bioactivity of the structures was tested with platelet adhesion experiments. Platelets adhered to and spread on printed vWF. These results indicate that printed vWF substrates are stable and functional in typical perfusion experiments, and thus provide a useful tool for studying thrombus formation in vitro.

Item Type: Article
Erschienen: 2012
Creators: Davydovskaya, Polina ; Janko, Marek ; Gaertner, Florian ; Ahmad, Zerkah ; Simsek, Özlem ; Maßberg, Steffen ; Stark, Robert W.
Type of entry: Bibliographie
Title: Blood platelet adhesion to printed von Willebrand factor
Language: English
Date: February 2012
Publisher: Wiley-VCH Verlag GmbH & Co. KGaA
Journal or Publication Title: Journal of Biomedical Materials Research Part A
Volume of the journal: 100
Issue Number: 2
DOI: 10.1002/jbm.a.33275
Abstract:

Von Willebrand factor (vWF), a glycoprotein in blood, mediates the adhesion of blood platelets and thus plays a crucial role in hemostasis and thrombosis. Functional coating of surfaces with vWF allows the investigation of in vitro adhesion of blood platelet. We used soft lithography to create a functional patterned substrate. vWF was printed on plasma-treated glass and mica surfaces, producing elongated network-like fibril structures. A minimum layer thickness of 3 nm was observed, corresponding to the height of a monolayer of vWF. The stability of the patterns was verified in a laminar fluid flow, and the bioactivity of the structures was tested with platelet adhesion experiments. Platelets adhered to and spread on printed vWF. These results indicate that printed vWF substrates are stable and functional in typical perfusion experiments, and thus provide a useful tool for studying thrombus formation in vitro.

Uncontrolled Keywords: von Willebrand factor, atomic force microscope, micro contact printing, blood platelet adhesion, lab on a chip
Divisions: 11 Department of Materials and Earth Sciences
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences > Material Science > Physics of Surfaces
Exzellenzinitiative
Exzellenzinitiative > Clusters of Excellence
Zentrale Einrichtungen
Exzellenzinitiative > Clusters of Excellence > Center of Smart Interfaces (CSI)
Date Deposited: 16 Jun 2014 09:21
Last Modified: 14 Jul 2020 11:27
PPN:
Funders: Funded by Clusters of Excellence Center of Smart Interfaces Darmstadt and Nanosystems Initiative Munich, NIM
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