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Synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor.

Rudolph, Martin Michael ; Vockenhuber, Michael-Paul ; Suess, Beatrix (2013)
Synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor.
In: Microbiology (Reading, England), 159
Article, Bibliographie

Abstract

We have demonstrated the portability of theophylline-dependent synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor. The riboswitches mediate dose-dependent, up to 260-fold activation of reporter gene expression. The riboswitch regulation offers a simple method since only a sequence of ~85 nucleotides has to be inserted between a transcriptional start site and the start codon and no further auxiliary factors are necessary. The promoters galP2, ermEp1 and SF14 worked well in concert with the riboswitches. They allowed theophylline-dependent expression of the heterologous β-glucuronidase reporter gene but also of an endogenous gene for the agarase dagA. The successful combination of all tested promoters with the riboswitches underlines the orthogonality of riboswitch regulation. We anticipate that any further natural or synthetic promoter can be combined with the riboswitch.

Item Type: Article
Erschienen: 2013
Creators: Rudolph, Martin Michael ; Vockenhuber, Michael-Paul ; Suess, Beatrix
Type of entry: Bibliographie
Title: Synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor.
Language: English
Date: 2013
Journal or Publication Title: Microbiology (Reading, England)
Volume of the journal: 159
Abstract:

We have demonstrated the portability of theophylline-dependent synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor. The riboswitches mediate dose-dependent, up to 260-fold activation of reporter gene expression. The riboswitch regulation offers a simple method since only a sequence of ~85 nucleotides has to be inserted between a transcriptional start site and the start codon and no further auxiliary factors are necessary. The promoters galP2, ermEp1 and SF14 worked well in concert with the riboswitches. They allowed theophylline-dependent expression of the heterologous β-glucuronidase reporter gene but also of an endogenous gene for the agarase dagA. The successful combination of all tested promoters with the riboswitches underlines the orthogonality of riboswitch regulation. We anticipate that any further natural or synthetic promoter can be combined with the riboswitch.

Divisions: 10 Department of Biology
10 Department of Biology > Synthetic Genetic Circuits (2020 renamed "Synthetic RNA biology)
Date Deposited: 21 May 2013 08:55
Last Modified: 10 Sep 2013 11:37
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