Rudolph, Martin Michael ; Vockenhuber, Michael-Paul ; Suess, Beatrix (2013):
Synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor.
In: Microbiology (Reading, England), 159, pp. 1416-1422. ISSN 1465-2080,
[Article]
Abstract
We have demonstrated the portability of theophylline-dependent synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor. The riboswitches mediate dose-dependent, up to 260-fold activation of reporter gene expression. The riboswitch regulation offers a simple method since only a sequence of ~85 nucleotides has to be inserted between a transcriptional start site and the start codon and no further auxiliary factors are necessary. The promoters galP2, ermEp1 and SF14 worked well in concert with the riboswitches. They allowed theophylline-dependent expression of the heterologous β-glucuronidase reporter gene but also of an endogenous gene for the agarase dagA. The successful combination of all tested promoters with the riboswitches underlines the orthogonality of riboswitch regulation. We anticipate that any further natural or synthetic promoter can be combined with the riboswitch.
| Item Type: | Article |
|---|---|
| Erschienen: | 2013 |
| Creators: | Rudolph, Martin Michael ; Vockenhuber, Michael-Paul ; Suess, Beatrix |
| Title: | Synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor. |
| Language: | English |
| Abstract: | We have demonstrated the portability of theophylline-dependent synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor. The riboswitches mediate dose-dependent, up to 260-fold activation of reporter gene expression. The riboswitch regulation offers a simple method since only a sequence of ~85 nucleotides has to be inserted between a transcriptional start site and the start codon and no further auxiliary factors are necessary. The promoters galP2, ermEp1 and SF14 worked well in concert with the riboswitches. They allowed theophylline-dependent expression of the heterologous β-glucuronidase reporter gene but also of an endogenous gene for the agarase dagA. The successful combination of all tested promoters with the riboswitches underlines the orthogonality of riboswitch regulation. We anticipate that any further natural or synthetic promoter can be combined with the riboswitch. |
| Journal or Publication Title: | Microbiology (Reading, England) |
| Volume of the journal: | 159 |
| Divisions: | 10 Department of Biology 10 Department of Biology > Synthetic Genetic Circuits |
| Date Deposited: | 21 May 2013 08:55 |
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