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Uptake of fluorescent nano beads into BY2-cells involves clathrin-dependent and clathrin-independent endocytosis.

Bandmann, Vera ; Müller, Jasmin Daniela ; Köhler, Tim ; Homann, Ulrike (2012)
Uptake of fluorescent nano beads into BY2-cells involves clathrin-dependent and clathrin-independent endocytosis.
In: FEBS letters, 586 (20)
Article, Bibliographie

Abstract

To follow endocytosis in BY-2 cells we made use of fluorescent nano beads. Beads with 20nm in diameter were internalised rapidly and accumulated partially in compartments also labelled by the endocytic marker FM4-64. Studies in BY-2 cells and protoplasts revealed that larger beads (100nm) were excluded from uptake into turgescent and plasmolysed cells while protoplasts were able to internalise beads with a diameter of up to 1000nm. Endocytosis of beads was only partially inhibited by the clathrin-specific inhibitor Ikarugamycin and strongly blocked by wortmannin. These results imply that uptake of beads involves clathrin-dependent and clathrin-independent endocytic mechanisms and supports the hypothesis that clathrin-independent endocytosis plays a general role in plants.

Item Type: Article
Erschienen: 2012
Creators: Bandmann, Vera ; Müller, Jasmin Daniela ; Köhler, Tim ; Homann, Ulrike
Type of entry: Bibliographie
Title: Uptake of fluorescent nano beads into BY2-cells involves clathrin-dependent and clathrin-independent endocytosis.
Language: English
Date: 2012
Journal or Publication Title: FEBS letters
Volume of the journal: 586
Issue Number: 20
Abstract:

To follow endocytosis in BY-2 cells we made use of fluorescent nano beads. Beads with 20nm in diameter were internalised rapidly and accumulated partially in compartments also labelled by the endocytic marker FM4-64. Studies in BY-2 cells and protoplasts revealed that larger beads (100nm) were excluded from uptake into turgescent and plasmolysed cells while protoplasts were able to internalise beads with a diameter of up to 1000nm. Endocytosis of beads was only partially inhibited by the clathrin-specific inhibitor Ikarugamycin and strongly blocked by wortmannin. These results imply that uptake of beads involves clathrin-dependent and clathrin-independent endocytic mechanisms and supports the hypothesis that clathrin-independent endocytosis plays a general role in plants.

Divisions: 10 Department of Biology
10 Department of Biology > Plant Cell Biology
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Date Deposited: 16 Oct 2012 08:35
Last Modified: 05 Mar 2013 10:03
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