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Aptamer-regulated expression of essential genes in yeast.

Suess, Beatrix and Entian, Karl-Dieter and Kötter, Peter and Weigand, Julia E. (2012):
Aptamer-regulated expression of essential genes in yeast.
In: Methods in molecular biology (Clifton, N.J.), 824, pp. 381-91. ISSN 1940-6029,
[Article]

Abstract

Conditional gene expression systems are important tools for the functional analysis of essential genes. Tetracycline (tc)-binding aptamers can be exploited as artificial riboswitches for the efficient control of gene expression by inserting them into the 5' untranslated region of an mRNA. The ligand-bound form of those mRNAs inhibits gene expression by interfering with translation initiation. In contrast to previous tc-dependent regulatory systems, where tc inhibits or activates transcription upon binding to the repressor protein TetR, the tc-binding aptamer system inhibits translation of the respective mRNA. We describe here a simple and powerful PCR-based strategy which allows easy tagging of any target gene in yeast using a tc aptamer-containing insertion cassette. The expression window can be adjusted with different promoters and protein synthesis is rapidly switched off.

Item Type: Article
Erschienen: 2012
Creators: Suess, Beatrix and Entian, Karl-Dieter and Kötter, Peter and Weigand, Julia E.
Title: Aptamer-regulated expression of essential genes in yeast.
Language: English
Abstract:

Conditional gene expression systems are important tools for the functional analysis of essential genes. Tetracycline (tc)-binding aptamers can be exploited as artificial riboswitches for the efficient control of gene expression by inserting them into the 5' untranslated region of an mRNA. The ligand-bound form of those mRNAs inhibits gene expression by interfering with translation initiation. In contrast to previous tc-dependent regulatory systems, where tc inhibits or activates transcription upon binding to the repressor protein TetR, the tc-binding aptamer system inhibits translation of the respective mRNA. We describe here a simple and powerful PCR-based strategy which allows easy tagging of any target gene in yeast using a tc aptamer-containing insertion cassette. The expression window can be adjusted with different promoters and protein synthesis is rapidly switched off.

Journal or Publication Title: Methods in molecular biology (Clifton, N.J.)
Journal volume: 824
Divisions: 10 Department of Biology
10 Department of Biology > Synthetic Genetic Circuits
10 Department of Biology > RNA Biochemistry
Date Deposited: 22 Feb 2012 09:57
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