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Clathrin-Independent Endocytosis Contributes to Uptake of Glucose into BY-2 Protoplasts.

Bandmann, Vera ; Homann, Ulrike (2012)
Clathrin-Independent Endocytosis Contributes to Uptake of Glucose into BY-2 Protoplasts.
In: The Plant journal : for cell and molecular biology, 70 (4)
Article, Bibliographie

Abstract

In eukaryotic cells several pathways for the internalisation of plasma membrane proteins and extracellular cargo molecules exist. These endocytic pathways can be grouped into clathrin-dependent and clathrin-independent endocytosis. While the former one has been described to be involved in a variety of cellular processes in plants, the latter one has so far only been identified in animal and yeast cells. Here we show that internalisation of fluorescent glucose into BY-2 cells leads to accumulation of the sugar into compartments of the endocytic pathway. This endocytic uptake of glucose was not blocked by ikarugamycin (IKA), an inhibitor of clathrin-dependent endocytosis, suggesting a role for clathrin-independent endocytosis in glucose uptake. Investigations of fusion and fission of single vesicles by membrane capacitance measurements revealed a stimulation of endocytic activity by extracellular glucose. Glucose stimulated fission of vesicles was not affected by addition of IKA or block of clathrin coat formation by transient over-expression HUB1 (C-terminal part of the clathrin heavy chain). These data demonstrate that clathrin-independent endocytosis does exist in plant cells. This pathway may represent a common mechanism for the uptake of external nutrients. © 2011 The Authors. The Plant Journal© 2011 Blackwell Publishing Ltd.

Item Type: Article
Erschienen: 2012
Creators: Bandmann, Vera ; Homann, Ulrike
Type of entry: Bibliographie
Title: Clathrin-Independent Endocytosis Contributes to Uptake of Glucose into BY-2 Protoplasts.
Language: English
Date: 2012
Journal or Publication Title: The Plant journal : for cell and molecular biology
Volume of the journal: 70
Issue Number: 4
Abstract:

In eukaryotic cells several pathways for the internalisation of plasma membrane proteins and extracellular cargo molecules exist. These endocytic pathways can be grouped into clathrin-dependent and clathrin-independent endocytosis. While the former one has been described to be involved in a variety of cellular processes in plants, the latter one has so far only been identified in animal and yeast cells. Here we show that internalisation of fluorescent glucose into BY-2 cells leads to accumulation of the sugar into compartments of the endocytic pathway. This endocytic uptake of glucose was not blocked by ikarugamycin (IKA), an inhibitor of clathrin-dependent endocytosis, suggesting a role for clathrin-independent endocytosis in glucose uptake. Investigations of fusion and fission of single vesicles by membrane capacitance measurements revealed a stimulation of endocytic activity by extracellular glucose. Glucose stimulated fission of vesicles was not affected by addition of IKA or block of clathrin coat formation by transient over-expression HUB1 (C-terminal part of the clathrin heavy chain). These data demonstrate that clathrin-independent endocytosis does exist in plant cells. This pathway may represent a common mechanism for the uptake of external nutrients. © 2011 The Authors. The Plant Journal© 2011 Blackwell Publishing Ltd.

Divisions: 10 Department of Biology > Plant Cell Biology
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10 Department of Biology
Date Deposited: 10 Jan 2012 08:26
Last Modified: 05 Mar 2013 09:57
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