TU Darmstadt / ULB / TUbiblio

The Nicotiana tabacum plasma membrane aquaporin NtAQP1 is mercury-insensitive and permeable for glycerol.

Biela, A. ; Grote, K. ; Otto, Beate ; Hoth, S. ; Hedrich, R. ; Kaldenhoff, Ralf (1999)
The Nicotiana tabacum plasma membrane aquaporin NtAQP1 is mercury-insensitive and permeable for glycerol.
In: The Plant journal : for cell and molecular biology, 18 (5)
Article, Bibliographie

Abstract

A new aquaporin from Nicotiana tabacum (cv. Samsun) was characterized. It shares sequence homology to the Arabidopsis thaliana PIP1 protein family. By two-phase partitioning and immunoblot analysis, plasma membrane localization could be demonstrated. The corresponding mRNA is highly abundant in roots and flowers, while it is rarely expressed in leaves and stems. Functional expression in Xenopus oocytes revealed that NtAQP1 can mediate glycerol transport in addition to water flow. However, NtAQP1 is impermeable for Na+, K+ and Cl- ions. The water permeability and selectivity could not be modulated by addition of mercurials or the activity of cAMP-dependent protein kinases.

Item Type: Article
Erschienen: 1999
Creators: Biela, A. ; Grote, K. ; Otto, Beate ; Hoth, S. ; Hedrich, R. ; Kaldenhoff, Ralf
Type of entry: Bibliographie
Title: The Nicotiana tabacum plasma membrane aquaporin NtAQP1 is mercury-insensitive and permeable for glycerol.
Language: English
Date: 1999
Journal or Publication Title: The Plant journal : for cell and molecular biology
Volume of the journal: 18
Issue Number: 5
Abstract:

A new aquaporin from Nicotiana tabacum (cv. Samsun) was characterized. It shares sequence homology to the Arabidopsis thaliana PIP1 protein family. By two-phase partitioning and immunoblot analysis, plasma membrane localization could be demonstrated. The corresponding mRNA is highly abundant in roots and flowers, while it is rarely expressed in leaves and stems. Functional expression in Xenopus oocytes revealed that NtAQP1 can mediate glycerol transport in addition to water flow. However, NtAQP1 is impermeable for Na+, K+ and Cl- ions. The water permeability and selectivity could not be modulated by addition of mercurials or the activity of cAMP-dependent protein kinases.

Divisions: 10 Department of Biology > Applied Plant Sciences
?? fb10_botanik ??
10 Department of Biology
Date Deposited: 31 Aug 2011 13:10
Last Modified: 05 Mar 2013 09:54
PPN:
Export:
Suche nach Titel in: TUfind oder in Google
Send an inquiry Send an inquiry

Options (only for editors)
Show editorial Details Show editorial Details