TU Darmstadt / ULB / TUbiblio

Chlorovirus-mediated membrane depolarization of Chlorella alters secondary active transport of solutes.

Agarkova, Irina ; Dunigan, David ; Gurnon, James ; Greiner, Timo ; Barres, Julia ; Thiel, Gerhard ; Etten, James L. van (2008)
Chlorovirus-mediated membrane depolarization of Chlorella alters secondary active transport of solutes.
In: Journal of virology, 82 (24)
Article, Bibliographie

Abstract

Paramecium bursaria chlorella virus 1 (PBCV-1) is the prototype of a family of large, double-stranded DNA, plaque-forming viruses that infect certain eukaryotic chlorella-like green algae from the genus Chlorovirus. PBCV-1 infection results in rapid host membrane depolarization and potassium ion release. One interesting feature of certain chloroviruses is that they code for functional potassium ion-selective channel proteins (Kcv) that are considered responsible for the host membrane depolarization and, as a consequence, the efflux of potassium ions. This report examines the relationship between cellular depolarization and solute uptake. Annotation of the virus host Chlorella strain NC64A genome revealed 482 putative transporter-encoding genes; 224 are secondary active transporters. Solute uptake experiments using seven radioactive compounds revealed that virus infection alters the transport of all the solutes. However, the degree of inhibition varied depending on the solute. Experiments with nystatin, a drug known to depolarize cell membranes, produced changes in solute uptake that are similar but not identical to those that occurred during virus infection. Therefore, these studies indicate that chlorovirus infection causes a rapid and sustained depolarization of the host plasma membrane and that this depolarization leads to the inhibition of secondary active transporters that changes solute uptake.

Item Type: Article
Erschienen: 2008
Creators: Agarkova, Irina ; Dunigan, David ; Gurnon, James ; Greiner, Timo ; Barres, Julia ; Thiel, Gerhard ; Etten, James L. van
Type of entry: Bibliographie
Title: Chlorovirus-mediated membrane depolarization of Chlorella alters secondary active transport of solutes.
Language: English
Date: 2008
Journal or Publication Title: Journal of virology
Volume of the journal: 82
Issue Number: 24
Abstract:

Paramecium bursaria chlorella virus 1 (PBCV-1) is the prototype of a family of large, double-stranded DNA, plaque-forming viruses that infect certain eukaryotic chlorella-like green algae from the genus Chlorovirus. PBCV-1 infection results in rapid host membrane depolarization and potassium ion release. One interesting feature of certain chloroviruses is that they code for functional potassium ion-selective channel proteins (Kcv) that are considered responsible for the host membrane depolarization and, as a consequence, the efflux of potassium ions. This report examines the relationship between cellular depolarization and solute uptake. Annotation of the virus host Chlorella strain NC64A genome revealed 482 putative transporter-encoding genes; 224 are secondary active transporters. Solute uptake experiments using seven radioactive compounds revealed that virus infection alters the transport of all the solutes. However, the degree of inhibition varied depending on the solute. Experiments with nystatin, a drug known to depolarize cell membranes, produced changes in solute uptake that are similar but not identical to those that occurred during virus infection. Therefore, these studies indicate that chlorovirus infection causes a rapid and sustained depolarization of the host plasma membrane and that this depolarization leads to the inhibition of secondary active transporters that changes solute uptake.

Divisions: 10 Department of Biology
?? fb10_botanik ??
10 Department of Biology > Plant Membrane Biophyscis (20.12.23 renamed in Biology of Algae and Protozoa)
Date Deposited: 21 Jun 2011 12:10
Last Modified: 20 Aug 2021 09:43
PPN:
Export:
Suche nach Titel in: TUfind oder in Google
Send an inquiry Send an inquiry

Options (only for editors)
Show editorial Details Show editorial Details