TU Darmstadt / ULB / TUbiblio

Integrin-dependent organization and bidirectional vesicular traffic at cytotoxic immune synapses.

Liu, Dongfang ; Bryceson, Yenan T. ; Meckel, Tobias ; Vasiliver-Shamis, Gaia ; Dustin, Michael L. ; Long, Eric O. (2009)
Integrin-dependent organization and bidirectional vesicular traffic at cytotoxic immune synapses.
In: Immunity, 31 (1)
Article, Bibliographie

Abstract

Cytotoxic lymphocytes kill target cells by releasing the content of secretory lysosomes at the immune synapse. To understand the dynamics and control of cytotoxic immune synapses, we imaged human primary, live natural killer cells on lipid bilayers carrying ligands of activation receptors. Formation of an organized synapse was dependent on the presence of the beta2 integrin ligand ICAM-1. Ligands of coactivation receptors 2B4 and NKG2D segregated into central and peripheral regions, respectively. Lysosomal protein LAMP-1 that was exocytosed during degranulation accumulated in a large and spatially stable cluster, which overlapped with a site of membrane internalization. Lysosomal compartments reached the plasma membrane at focal points adjacent to centrally accumulated LAMP-1. Imaging of fixed cells revealed that perforin-containing granules were juxtaposed to an intracellular compartment where exocytosed LAMP-1 was retrieved. Thus, cytotoxic immune synapses include a central region of bidirectional vesicular traffic, which is controlled by integrin signaling.

Item Type: Article
Erschienen: 2009
Creators: Liu, Dongfang ; Bryceson, Yenan T. ; Meckel, Tobias ; Vasiliver-Shamis, Gaia ; Dustin, Michael L. ; Long, Eric O.
Type of entry: Bibliographie
Title: Integrin-dependent organization and bidirectional vesicular traffic at cytotoxic immune synapses.
Language: English
Date: 2009
Journal or Publication Title: Immunity
Volume of the journal: 31
Issue Number: 1
Abstract:

Cytotoxic lymphocytes kill target cells by releasing the content of secretory lysosomes at the immune synapse. To understand the dynamics and control of cytotoxic immune synapses, we imaged human primary, live natural killer cells on lipid bilayers carrying ligands of activation receptors. Formation of an organized synapse was dependent on the presence of the beta2 integrin ligand ICAM-1. Ligands of coactivation receptors 2B4 and NKG2D segregated into central and peripheral regions, respectively. Lysosomal protein LAMP-1 that was exocytosed during degranulation accumulated in a large and spatially stable cluster, which overlapped with a site of membrane internalization. Lysosomal compartments reached the plasma membrane at focal points adjacent to centrally accumulated LAMP-1. Imaging of fixed cells revealed that perforin-containing granules were juxtaposed to an intracellular compartment where exocytosed LAMP-1 was retrieved. Thus, cytotoxic immune synapses include a central region of bidirectional vesicular traffic, which is controlled by integrin signaling.

Divisions: 10 Department of Biology
?? fb10_botanik ??
10 Department of Biology > Plant Membrane Biophyscis (20.12.23 renamed in Biology of Algae and Protozoa)
10 Department of Biology > Membrane Dynamics
Date Deposited: 06 Jun 2011 13:34
Last Modified: 05 Mar 2013 09:48
PPN:
Export:
Suche nach Titel in: TUfind oder in Google
Send an inquiry Send an inquiry

Options (only for editors)
Show editorial Details Show editorial Details