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Imaging protein-protein interactions by Förster resonance energy transfer (FRET) microscopy in live cells

Brzostowski, Joseph A. ; Meckel, Tobias ; Hong, Jiang ; Chen, Alice ; Jin, Tian (2009)
Imaging protein-protein interactions by Förster resonance energy transfer (FRET) microscopy in live cells.
In: Current Protocols in Protein Science, 56 (1)
doi: 10.1002/0471140864.ps1905s56
Article, Bibliographie

Abstract

This unit describes an acceptor-sensitized emission FRET method using a confocal microscope for image acquisition. In contrast to acceptor photobleaching, which is an end-point assay that destroys the acceptor fluorophore, the sensitized emission method is amenable for FRET measurements in live cells and can be used to measure changes in FRET efficiency over time. The purpose of this unit is to provide a basic starting point for understanding and performing the sensitized emission method with a simple teaching tool for live-cell imaging. References that discuss the vagaries of acquiring and analyzing FRET between individually tagged molecules are provided.

Item Type: Article
Erschienen: 2009
Creators: Brzostowski, Joseph A. ; Meckel, Tobias ; Hong, Jiang ; Chen, Alice ; Jin, Tian
Type of entry: Bibliographie
Title: Imaging protein-protein interactions by Förster resonance energy transfer (FRET) microscopy in live cells
Language: English
Date: 1 April 2009
Publisher: Wiley
Journal or Publication Title: Current Protocols in Protein Science
Volume of the journal: 56
Issue Number: 1
DOI: 10.1002/0471140864.ps1905s56
Abstract:

This unit describes an acceptor-sensitized emission FRET method using a confocal microscope for image acquisition. In contrast to acceptor photobleaching, which is an end-point assay that destroys the acceptor fluorophore, the sensitized emission method is amenable for FRET measurements in live cells and can be used to measure changes in FRET efficiency over time. The purpose of this unit is to provide a basic starting point for understanding and performing the sensitized emission method with a simple teaching tool for live-cell imaging. References that discuss the vagaries of acquiring and analyzing FRET between individually tagged molecules are provided.

Divisions: 10 Department of Biology
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10 Department of Biology > Plant Membrane Biophyscis (20.12.23 renamed in Biology of Algae and Protozoa)
10 Department of Biology > Membrane Dynamics
Date Deposited: 06 Jun 2011 13:32
Last Modified: 16 Jun 2020 11:20
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