TU Darmstadt / ULB / TUbiblio

Functional hammerhead ribozymes naturally encoded in the genome of Arabidopsis thaliana.

Przybilski, Rita ; Gräf, Stefan ; Lescoute, Aurélie ; Nellen, Wolfgang ; Westhof, Eric ; Steger, Gerhard ; Hammann, Christian (2005)
Functional hammerhead ribozymes naturally encoded in the genome of Arabidopsis thaliana.
In: The Plant cell, 17 (7)
Article, Bibliographie

Abstract

The hammerhead ribozyme (HHRz) is an autocatalytic RNA motif found in subviral plant pathogens and transcripts of repetitive DNA sequences in animals. Here, we report the discovery and characterization of unique HHRzs encoded in a plant genome. Two novel sequences were identified on chromosome IV of Arabidopsis thaliana in a database search, which took into account recently defined structural requirements. The HHRzs are expressed in several tissues and coexist in vivo as both cleaved and noncleaved species. In vitro, both sequences cleave efficiently at physiological Mg(2+) concentrations, indicative of functional loop-loop interactions. Kinetic analysis of loop nucleotide variants was used to determine a three-dimensional model of these tertiary interactions. Based on these results, on the lack of infectivity of hammerhead-carrying viroids in Arabidopsis, and on extensive sequence comparisons, we propose that the ribozyme sequences did not invade this plant by horizontal transfer but have evolved independently to perform a specific, yet unidentified, biological function.

Item Type: Article
Erschienen: 2005
Creators: Przybilski, Rita ; Gräf, Stefan ; Lescoute, Aurélie ; Nellen, Wolfgang ; Westhof, Eric ; Steger, Gerhard ; Hammann, Christian
Type of entry: Bibliographie
Title: Functional hammerhead ribozymes naturally encoded in the genome of Arabidopsis thaliana.
Language: English
Date: 2005
Journal or Publication Title: The Plant cell
Volume of the journal: 17
Issue Number: 7
Abstract:

The hammerhead ribozyme (HHRz) is an autocatalytic RNA motif found in subviral plant pathogens and transcripts of repetitive DNA sequences in animals. Here, we report the discovery and characterization of unique HHRzs encoded in a plant genome. Two novel sequences were identified on chromosome IV of Arabidopsis thaliana in a database search, which took into account recently defined structural requirements. The HHRzs are expressed in several tissues and coexist in vivo as both cleaved and noncleaved species. In vitro, both sequences cleave efficiently at physiological Mg(2+) concentrations, indicative of functional loop-loop interactions. Kinetic analysis of loop nucleotide variants was used to determine a three-dimensional model of these tertiary interactions. Based on these results, on the lack of infectivity of hammerhead-carrying viroids in Arabidopsis, and on extensive sequence comparisons, we propose that the ribozyme sequences did not invade this plant by horizontal transfer but have evolved independently to perform a specific, yet unidentified, biological function.

Divisions: 10 Department of Biology
?? fb10_mikrobiologie ??
10 Department of Biology > Ribogenetics
Date Deposited: 29 Jul 2010 12:25
Last Modified: 05 Mar 2013 09:34
PPN:
Export:
Suche nach Titel in: TUfind oder in Google
Send an inquiry Send an inquiry

Options (only for editors)
Show editorial Details Show editorial Details