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High-Scale 3D-Bioprinting Platform for the Automated Production of Vascularized Organs-on-a-Chip

Fritschen, Anna ; Lindner, Nils ; Scholpp, Sebastian ; Richthof, Philipp ; Dietz, Jonas ; Linke, Philipp ; Guttenberg, Zeno ; Blaeser, Andreas (2024)
High-Scale 3D-Bioprinting Platform for the Automated Production of Vascularized Organs-on-a-Chip.
In: Advanced Healthcare Materials
doi: 10.1002/adhm.202304028
Article, Bibliographie

Abstract

Abstract 3D bioprinting possesses the potential to revolutionize contemporary methodologies for fabricating tissue models employed in pharmaceutical research and experimental investigations. This is enhanced by combining bioprinting with advanced organs-on-a-chip (OOCs), which includes a complex arrangement of multiple cell types representing organ-specific cells, connective tissue, and vasculature. However, both OOCs and bioprinting so far demand a high degree of manual intervention, thereby impeding efficiency and inhibiting scalability to meet technological requirements. Through the combination of drop-on-demand bioprinting with robotic handling of microfluidic chips, a print procedure is achieved that is proficient in managing three distinct tissue models on a chip within only a minute, as well as capable of consecutively processing numerous OOCs without manual intervention. This process rests upon the development of a post-printing sealable microfluidic chip, that is compatible with different types of 3D-bioprinters and easily connected to a perfusion system. The capabilities of the automized bioprint process are showcased through the creation of a multicellular and vascularized liver carcinoma model on the chip. The process achieves full vascularization and stable microvascular network formation over 14 days of culture time, with pronounced spheroidal cell growth and albumin secretion of HepG2 serving as a representative cell model.

Item Type: Article
Erschienen: 2024
Creators: Fritschen, Anna ; Lindner, Nils ; Scholpp, Sebastian ; Richthof, Philipp ; Dietz, Jonas ; Linke, Philipp ; Guttenberg, Zeno ; Blaeser, Andreas
Type of entry: Bibliographie
Title: High-Scale 3D-Bioprinting Platform for the Automated Production of Vascularized Organs-on-a-Chip
Language: English
Date: 2024
Place of Publication: Weinheim
Publisher: Wiley-VCH
Journal or Publication Title: Advanced Healthcare Materials
Collation: 11 Seiten
DOI: 10.1002/adhm.202304028
URL / URN: https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.2023040...
Abstract:

Abstract 3D bioprinting possesses the potential to revolutionize contemporary methodologies for fabricating tissue models employed in pharmaceutical research and experimental investigations. This is enhanced by combining bioprinting with advanced organs-on-a-chip (OOCs), which includes a complex arrangement of multiple cell types representing organ-specific cells, connective tissue, and vasculature. However, both OOCs and bioprinting so far demand a high degree of manual intervention, thereby impeding efficiency and inhibiting scalability to meet technological requirements. Through the combination of drop-on-demand bioprinting with robotic handling of microfluidic chips, a print procedure is achieved that is proficient in managing three distinct tissue models on a chip within only a minute, as well as capable of consecutively processing numerous OOCs without manual intervention. This process rests upon the development of a post-printing sealable microfluidic chip, that is compatible with different types of 3D-bioprinters and easily connected to a perfusion system. The capabilities of the automized bioprint process are showcased through the creation of a multicellular and vascularized liver carcinoma model on the chip. The process achieves full vascularization and stable microvascular network formation over 14 days of culture time, with pronounced spheroidal cell growth and albumin secretion of HepG2 serving as a representative cell model.

Uncontrolled Keywords: bioprinting, organ-on-a-chip, robotics, vascularization
Identification Number: Artikel-ID: 2304028
Additional Information:

EarlyView Article

Divisions: 16 Department of Mechanical Engineering
16 Department of Mechanical Engineering > Institute of Printing Science and Technology (IDD)
Date Deposited: 16 Apr 2024 06:21
Last Modified: 16 Apr 2024 06:21
PPN: 517168375
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