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Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays.

Elter, Adrian ; Bock, Tina ; Spiehl, Dieter ; Russo, Giulio ; Hinz, Steffen C. ; Bitsch, Sebastian ; Baum, Eva ; Langhans, Markus ; Meckel, Tobias ; Dörsam, Edgar ; Kolmar, Harald ; Schwall, Gerhard (2021)
Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays.
In: Scientific reports, 11 (1)
doi: 10.1038/s41598-021-87072-7
Artikel, Bibliographie

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Kurzbeschreibung (Abstract)

Since the pandemic outbreak of Covid-19 in December 2019, several lateral flow assay (LFA) devices were developed to enable the constant monitoring of regional and global infection processes. Additionally, innumerable lateral flow test devices are frequently used for determination of different clinical parameters, food safety, and environmental factors. Since common LFAs rely on non-biodegradable nitrocellulose membranes, we focused on their replacement by cellulose-composed, biodegradable papers. We report the development of cellulose paper-based lateral flow immunoassays using a carbohydrate-binding module-fused to detection antibodies. Studies regarding the protein binding capacity and potential protein wash-off effects on cellulose paper demonstrated a 2.7-fold protein binding capacity of CBM-fused antibody fragments compared to the sole antibody fragment. Furthermore, this strategy improved the spatial retention of CBM-fused detection antibodies to the test area, which resulted in an enhanced sensitivity and improved overall LFA-performance compared to the naked detection antibody. CBM-assisted antibodies were validated by implementation into two model lateral flow test devices (pregnancy detection and the detection of SARS-CoV-2 specific antibodies). The CBM-assisted pregnancy LFA demonstrated sensitive detection of human gonadotropin (hCG) in synthetic urine and the CBM-assisted Covid-19 antibody LFA was able to detect SARS-CoV-2 specific antibodies present in serum. Our findings pave the way to the more frequent use of cellulose-based papers instead of nitrocellulose in LFA devices and thus potentially improve the sustainability in the field of POC diagnostics.

Typ des Eintrags: Artikel
Erschienen: 2021
Autor(en): Elter, Adrian ; Bock, Tina ; Spiehl, Dieter ; Russo, Giulio ; Hinz, Steffen C. ; Bitsch, Sebastian ; Baum, Eva ; Langhans, Markus ; Meckel, Tobias ; Dörsam, Edgar ; Kolmar, Harald ; Schwall, Gerhard
Art des Eintrags: Bibliographie
Titel: Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays.
Sprache: Englisch
Publikationsjahr: 12 April 2021
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Scientific reports
Jahrgang/Volume einer Zeitschrift: 11
(Heft-)Nummer: 1
DOI: 10.1038/s41598-021-87072-7
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Kurzbeschreibung (Abstract):

Since the pandemic outbreak of Covid-19 in December 2019, several lateral flow assay (LFA) devices were developed to enable the constant monitoring of regional and global infection processes. Additionally, innumerable lateral flow test devices are frequently used for determination of different clinical parameters, food safety, and environmental factors. Since common LFAs rely on non-biodegradable nitrocellulose membranes, we focused on their replacement by cellulose-composed, biodegradable papers. We report the development of cellulose paper-based lateral flow immunoassays using a carbohydrate-binding module-fused to detection antibodies. Studies regarding the protein binding capacity and potential protein wash-off effects on cellulose paper demonstrated a 2.7-fold protein binding capacity of CBM-fused antibody fragments compared to the sole antibody fragment. Furthermore, this strategy improved the spatial retention of CBM-fused detection antibodies to the test area, which resulted in an enhanced sensitivity and improved overall LFA-performance compared to the naked detection antibody. CBM-assisted antibodies were validated by implementation into two model lateral flow test devices (pregnancy detection and the detection of SARS-CoV-2 specific antibodies). The CBM-assisted pregnancy LFA demonstrated sensitive detection of human gonadotropin (hCG) in synthetic urine and the CBM-assisted Covid-19 antibody LFA was able to detect SARS-CoV-2 specific antibodies present in serum. Our findings pave the way to the more frequent use of cellulose-based papers instead of nitrocellulose in LFA devices and thus potentially improve the sustainability in the field of POC diagnostics.

ID-Nummer: pmid:33846482
Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Membrane Dynamics
16 Fachbereich Maschinenbau
16 Fachbereich Maschinenbau > Institut für Druckmaschinen und Druckverfahren (IDD)
07 Fachbereich Chemie
07 Fachbereich Chemie > Clemens-Schöpf-Institut > Fachgebiet Biochemie
07 Fachbereich Chemie > Clemens-Schöpf-Institut
Hinterlegungsdatum: 19 Apr 2021 12:57
Letzte Änderung: 03 Jul 2024 02:51
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