Elter, Adrian ; Bock, Tina ; Spiehl, Dieter ; Russo, Giulio ; Hinz, Steffen C. ; Bitsch, Sebastian ; Baum, Eva ; Langhans, Markus ; Meckel, Tobias ; Dörsam, Edgar ; Kolmar, Harald ; Schwall, Gerhard (2021)
Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays.
In: Scientific reports, 11 (1)
doi: 10.1038/s41598-021-87072-7
Article, Bibliographie
This is the latest version of this item.
Abstract
Since the pandemic outbreak of Covid-19 in December 2019, several lateral flow assay (LFA) devices were developed to enable the constant monitoring of regional and global infection processes. Additionally, innumerable lateral flow test devices are frequently used for determination of different clinical parameters, food safety, and environmental factors. Since common LFAs rely on non-biodegradable nitrocellulose membranes, we focused on their replacement by cellulose-composed, biodegradable papers. We report the development of cellulose paper-based lateral flow immunoassays using a carbohydrate-binding module-fused to detection antibodies. Studies regarding the protein binding capacity and potential protein wash-off effects on cellulose paper demonstrated a 2.7-fold protein binding capacity of CBM-fused antibody fragments compared to the sole antibody fragment. Furthermore, this strategy improved the spatial retention of CBM-fused detection antibodies to the test area, which resulted in an enhanced sensitivity and improved overall LFA-performance compared to the naked detection antibody. CBM-assisted antibodies were validated by implementation into two model lateral flow test devices (pregnancy detection and the detection of SARS-CoV-2 specific antibodies). The CBM-assisted pregnancy LFA demonstrated sensitive detection of human gonadotropin (hCG) in synthetic urine and the CBM-assisted Covid-19 antibody LFA was able to detect SARS-CoV-2 specific antibodies present in serum. Our findings pave the way to the more frequent use of cellulose-based papers instead of nitrocellulose in LFA devices and thus potentially improve the sustainability in the field of POC diagnostics.
| Item Type: | Article |
|---|---|
| Erschienen: | 2021 |
| Creators: | Elter, Adrian ; Bock, Tina ; Spiehl, Dieter ; Russo, Giulio ; Hinz, Steffen C. ; Bitsch, Sebastian ; Baum, Eva ; Langhans, Markus ; Meckel, Tobias ; Dörsam, Edgar ; Kolmar, Harald ; Schwall, Gerhard |
| Type of entry: | Bibliographie |
| Title: | Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays. |
| Language: | English |
| Date: | 12 April 2021 |
| Journal or Publication Title: | Scientific reports |
| Volume of the journal: | 11 |
| Issue Number: | 1 |
| DOI: | 10.1038/s41598-021-87072-7 |
| Corresponding Links: | |
| Abstract: | Since the pandemic outbreak of Covid-19 in December 2019, several lateral flow assay (LFA) devices were developed to enable the constant monitoring of regional and global infection processes. Additionally, innumerable lateral flow test devices are frequently used for determination of different clinical parameters, food safety, and environmental factors. Since common LFAs rely on non-biodegradable nitrocellulose membranes, we focused on their replacement by cellulose-composed, biodegradable papers. We report the development of cellulose paper-based lateral flow immunoassays using a carbohydrate-binding module-fused to detection antibodies. Studies regarding the protein binding capacity and potential protein wash-off effects on cellulose paper demonstrated a 2.7-fold protein binding capacity of CBM-fused antibody fragments compared to the sole antibody fragment. Furthermore, this strategy improved the spatial retention of CBM-fused detection antibodies to the test area, which resulted in an enhanced sensitivity and improved overall LFA-performance compared to the naked detection antibody. CBM-assisted antibodies were validated by implementation into two model lateral flow test devices (pregnancy detection and the detection of SARS-CoV-2 specific antibodies). The CBM-assisted pregnancy LFA demonstrated sensitive detection of human gonadotropin (hCG) in synthetic urine and the CBM-assisted Covid-19 antibody LFA was able to detect SARS-CoV-2 specific antibodies present in serum. Our findings pave the way to the more frequent use of cellulose-based papers instead of nitrocellulose in LFA devices and thus potentially improve the sustainability in the field of POC diagnostics. |
| Identification Number: | pmid:33846482 |
| Divisions: | 10 Department of Biology 10 Department of Biology > Membrane Dynamics 16 Department of Mechanical Engineering 16 Department of Mechanical Engineering > Institute of Printing Science and Technology (IDD) 07 Department of Chemistry 07 Department of Chemistry > Clemens-Schöpf-Institut > Fachgebiet Biochemie 07 Department of Chemistry > Clemens-Schöpf-Institut |
| Date Deposited: | 19 Apr 2021 12:57 |
| Last Modified: | 03 Jul 2024 02:51 |
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| Suche nach Titel in: | TUfind oder in Google |
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Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays. (deposited 03 May 2022 11:22)
- Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays. (deposited 19 Apr 2021 12:57) [Currently Displayed]
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