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Linker engineering in the context of synthetic protein switches and sensors.

Gräwe, Alexander ; Stein, Viktor (2020)
Linker engineering in the context of synthetic protein switches and sensors.
In: Trends in biotechnology
doi: 10.1016/j.tibtech.2020.11.007
Article, Bibliographie

Abstract

Linkers play critical roles in the construction of synthetic protein switches and sensors as they functionally couple a receptor with an actuator. With an increasing number of molecular toolboxes and experimental strategies becoming available that can be applied to engineer protein switches and sensors with tailored response functions, optimising the connecting linkers remains an idiosyncratic and empiric process. This review aims to provide an in-depth analysis of linker motifs, the biophysical properties they confer, and how they impact the performance of synthetic protein switches and sensors while identifying trends, mechanisms, and strategies that underlie the most potent switches and sensors.

Item Type: Article
Erschienen: 2020
Creators: Gräwe, Alexander ; Stein, Viktor
Type of entry: Bibliographie
Title: Linker engineering in the context of synthetic protein switches and sensors.
Language: English
Date: 5 December 2020
Journal or Publication Title: Trends in biotechnology
DOI: 10.1016/j.tibtech.2020.11.007
Abstract:

Linkers play critical roles in the construction of synthetic protein switches and sensors as they functionally couple a receptor with an actuator. With an increasing number of molecular toolboxes and experimental strategies becoming available that can be applied to engineer protein switches and sensors with tailored response functions, optimising the connecting linkers remains an idiosyncratic and empiric process. This review aims to provide an in-depth analysis of linker motifs, the biophysical properties they confer, and how they impact the performance of synthetic protein switches and sensors while identifying trends, mechanisms, and strategies that underlie the most potent switches and sensors.

Identification Number: pmid:33293101
Divisions: 10 Department of Biology
10 Department of Biology > Protein Engineering of Ion Conducting Nanopores
Date Deposited: 14 Dec 2020 13:15
Last Modified: 14 Dec 2020 13:15
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