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Ultrasensitive and selective copper(II) detection: introducing a bioinspired and robust sensor

Müller, Lena K. ; Duznovic, Ivana ; Tietze, Daniel ; Weber, Wadim ; Ali, Mubarak ; Stein, Viktor ; Ensinger, Wolfgang ; Tietze, Alesia A. (2020)
Ultrasensitive and selective copper(II) detection: introducing a bioinspired and robust sensor.
In: Chemistry – A European Journal, 26 (39)
doi: 10.1002/chem.202001160
Article, Bibliographie

Abstract

We report a nanopore-based Cu(II) sensing system which allows for an ultrasensitive and selective detection of Cu(II) with the possibility for a broad range of applications, i.e. medical diagnostics. A fluorescent ATCUN-like peptide 5/6-FAM-Dap-β-Ala-His is employed to selectively bind Cu(II) ions in the presence of Ni(II) and Zn(II) and was crafted into ion track-etched nanopores. Upon Cu(II) binding the fluorescence of the peptide sensor is quenched, permitting the detection of Cu(II) in solution. The ion transport characteristics of peptide-modified nanopore are shown to be extremely sensitive and selective towards Cu(II) allowing to sense femtomolar Cu(II) concentrations in human urine mimics. Washing with EDTA fully restores the Cu(II)-binding properties of the sensor, enabling multiple repetitive measurements. The robustness of the system clearly has the potential to be further developed into an easy-to-use, lab-on-chip Cu(II)-sensing device, which will be of great importance for bedside diagnosis and monitor of Cu(II) levels in patients with copper dysfunctional homeostasis.

Item Type: Article
Erschienen: 2020
Creators: Müller, Lena K. ; Duznovic, Ivana ; Tietze, Daniel ; Weber, Wadim ; Ali, Mubarak ; Stein, Viktor ; Ensinger, Wolfgang ; Tietze, Alesia A.
Type of entry: Bibliographie
Title: Ultrasensitive and selective copper(II) detection: introducing a bioinspired and robust sensor
Language: English
Date: 14 July 2020
Publisher: Wiley-VCH
Journal or Publication Title: Chemistry – A European Journal
Volume of the journal: 26
Issue Number: 39
DOI: 10.1002/chem.202001160
Abstract:

We report a nanopore-based Cu(II) sensing system which allows for an ultrasensitive and selective detection of Cu(II) with the possibility for a broad range of applications, i.e. medical diagnostics. A fluorescent ATCUN-like peptide 5/6-FAM-Dap-β-Ala-His is employed to selectively bind Cu(II) ions in the presence of Ni(II) and Zn(II) and was crafted into ion track-etched nanopores. Upon Cu(II) binding the fluorescence of the peptide sensor is quenched, permitting the detection of Cu(II) in solution. The ion transport characteristics of peptide-modified nanopore are shown to be extremely sensitive and selective towards Cu(II) allowing to sense femtomolar Cu(II) concentrations in human urine mimics. Washing with EDTA fully restores the Cu(II)-binding properties of the sensor, enabling multiple repetitive measurements. The robustness of the system clearly has the potential to be further developed into an easy-to-use, lab-on-chip Cu(II)-sensing device, which will be of great importance for bedside diagnosis and monitor of Cu(II) levels in patients with copper dysfunctional homeostasis.

Uncontrolled Keywords: Liebig Fellowship of the Fonds der Chemischen Industrie, LOEWE project iNAPO - Ministry of Higher education, Research and the Arts (HMWK) of the Hessen state, Knut & Alice Wallenberg Foundation, ATCUN, copper, fluorescence, nanopores, sensors
Identification Number: pmid:32196774
Divisions: 10 Department of Biology
10 Department of Biology > Protein Engineering of Ion Conducting Nanopores
11 Department of Materials and Earth Sciences
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences > Material Science > Material Analytics
Date Deposited: 25 Mar 2020 07:46
Last Modified: 18 Nov 2020 13:41
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