Masedunskas, Andrius ; Appaduray, Mark A. ; Lucas, Christine A. ; Cagigas, María Lastra ; Heydecker, Marco ; Holliday, Mira ; Meiring, Joyce ; Hook, Jeff ; Kee, Anthony ; White, Melissa ; Thomas, Paul ; Zhang, Yingfan ; Adelstein, Robert S. ; Meckel, Tobias ; Böcking, Till ; Weigert, Roberto ; Bryce, Nicole S. ; Gunning, Peter W. ; Hardeman, Edna C. (2018)
Parallel assembly of actin and tropomyosin but not myosin II during de novo actin filament formation in live mice.
In: Journal of cell science, 131 (6)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Many actin filaments in animal cells are co-polymers of actin and tropomyosin. For many of these co-polymers, non-muscle myosin II associates to establish a contractile network. However, the temporal relationship of these 3 proteins in theassembly of actin filaments is not known. Intravital subcellular microscopy of secretory granule exocytosis allows the visualisation and quantitation of the formation of an actin scaffold in real time with the added advantage that it occurs in a living mammal, under physiological conditions. We used this model system to investigate theassembly of actin, tropomyosin Tpm3.1 and myosin IIA on secretory granules in mouse salivary glands. Blocking actin polymerization with cytochalasin D revealed that Tpm3.1 assembly is dependent on actin assembly. We used time-lapse imaging to determine the timing of the appearance of the actin filament reporter LifeAct-RFP and of Tpm3.1-mNeonGreen on secretory granules in LifeAct-RFP transgenic, Tpm3.1-mNeonGreen and myosin IIA-GFP knock-in mice. Our findings are consistent with the addition of tropomyosin to actin filaments shortly after the initiation of actin filament nucleation, followed by myosin IIA recruitment.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2018 |
| Autor(en): | Masedunskas, Andrius ; Appaduray, Mark A. ; Lucas, Christine A. ; Cagigas, María Lastra ; Heydecker, Marco ; Holliday, Mira ; Meiring, Joyce ; Hook, Jeff ; Kee, Anthony ; White, Melissa ; Thomas, Paul ; Zhang, Yingfan ; Adelstein, Robert S. ; Meckel, Tobias ; Böcking, Till ; Weigert, Roberto ; Bryce, Nicole S. ; Gunning, Peter W. ; Hardeman, Edna C. |
| Art des Eintrags: | Bibliographie |
| Titel: | Parallel assembly of actin and tropomyosin but not myosin II during de novo actin filament formation in live mice. |
| Sprache: | Englisch |
| Publikationsjahr: | März 2018 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of cell science |
| Jahrgang/Volume einer Zeitschrift: | 131 |
| (Heft-)Nummer: | 6 |
| Kurzbeschreibung (Abstract): | Many actin filaments in animal cells are co-polymers of actin and tropomyosin. For many of these co-polymers, non-muscle myosin II associates to establish a contractile network. However, the temporal relationship of these 3 proteins in theassembly of actin filaments is not known. Intravital subcellular microscopy of secretory granule exocytosis allows the visualisation and quantitation of the formation of an actin scaffold in real time with the added advantage that it occurs in a living mammal, under physiological conditions. We used this model system to investigate theassembly of actin, tropomyosin Tpm3.1 and myosin IIA on secretory granules in mouse salivary glands. Blocking actin polymerization with cytochalasin D revealed that Tpm3.1 assembly is dependent on actin assembly. We used time-lapse imaging to determine the timing of the appearance of the actin filament reporter LifeAct-RFP and of Tpm3.1-mNeonGreen on secretory granules in LifeAct-RFP transgenic, Tpm3.1-mNeonGreen and myosin IIA-GFP knock-in mice. Our findings are consistent with the addition of tropomyosin to actin filaments shortly after the initiation of actin filament nucleation, followed by myosin IIA recruitment. |
| ID-Nummer: | pmid:29487177 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Membrane Dynamics |
| Hinterlegungsdatum: | 07 Mär 2018 09:02 |
| Letzte Änderung: | 05 Apr 2018 06:36 |
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