Ohuchi, Shoji ; Suess, Beatrix (2018)
Altered stoichiometry of an evolved RNA aptamer.
In: RNA (New York, N.Y.), 24 (4)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Inhibitory aptamers against a protein are promising as antagonistic reagents and repressive genetic components. Typically, improvement of such aptamers is achieved by acquiring higher binding affinity. Here, we report an alternative mechanism for the improvement of aptamer activity. Recently, we reported a transcriptional activator based on an inhibitory RNA aptamer against lambda cI repressor. We improved the aptamer through in vitro selection (SELEX) from a randomly mutagenized aptamer pool, followed by in vivo screening and truncation. Biochemical analyses indicated that the activity improvement was achieved by alteration of the complex formation stoichiometry, rather than by higher affinity or expression. Our results suggest an alternative strategy for improving aptamer activity.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2018 |
| Autor(en): | Ohuchi, Shoji ; Suess, Beatrix |
| Art des Eintrags: | Bibliographie |
| Titel: | Altered stoichiometry of an evolved RNA aptamer. |
| Sprache: | Englisch |
| Publikationsjahr: | April 2018 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | RNA (New York, N.Y.) |
| Jahrgang/Volume einer Zeitschrift: | 24 |
| (Heft-)Nummer: | 4 |
| Kurzbeschreibung (Abstract): | Inhibitory aptamers against a protein are promising as antagonistic reagents and repressive genetic components. Typically, improvement of such aptamers is achieved by acquiring higher binding affinity. Here, we report an alternative mechanism for the improvement of aptamer activity. Recently, we reported a transcriptional activator based on an inhibitory RNA aptamer against lambda cI repressor. We improved the aptamer through in vitro selection (SELEX) from a randomly mutagenized aptamer pool, followed by in vivo screening and truncation. Biochemical analyses indicated that the activity improvement was achieved by alteration of the complex formation stoichiometry, rather than by higher affinity or expression. Our results suggest an alternative strategy for improving aptamer activity. |
| ID-Nummer: | pmid:29284756 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Synthetic Genetic Circuits (2020 umbenannt in "Synthetic RNA biology") |
| Hinterlegungsdatum: | 03 Jan 2018 10:33 |
| Letzte Änderung: | 05 Apr 2018 06:34 |
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