Babel, Laura ; Kruse, Larissa ; Bump, Steven ; Langhans, Markus ; Meckel, Tobias (2017)
Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions.
In: BMC Research Notes, 2017, 10 (1)
doi: 10.1186/s13104-017-3032-8
Artikel, Zweitveröffentlichung, Verlagsversion
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Kurzbeschreibung (Abstract)
Objective: Adhesion of cells to the extracellular matrix is facilitated by integrin receptors. We recently found that a nanoscale organization of plasma membrane located integrins containing the β1 subunit is responsible for an enhanced radio-resistance in 3D cultured cells over cells grown in 2D. While ionizing radiation is known to have broad effects on the lipid composition of the plasma membrane and their organization in lipid-rafts, it is not clear whether the effects of ionizing radiation on the nanoscale clustering of integrins is lipid-raft dependent. Results: Using single molecule microscopy we can show that β1 integrins colocalize with cholesterol in lipid-rafts. Ionizing radiation, as an extrinsic stressor, causes the separation of β1 integrins from cholesterol lipid raft suggesting that the effects of ionizing radiation on the clustering of β1 integrins are lipid-raft independent.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2017 |
| Autor(en): | Babel, Laura ; Kruse, Larissa ; Bump, Steven ; Langhans, Markus ; Meckel, Tobias |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions |
| Sprache: | Englisch |
| Publikationsjahr: | 2017 |
| Publikationsdatum der Erstveröffentlichung: | 2017 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | BMC Research Notes |
| Jahrgang/Volume einer Zeitschrift: | 10 |
| (Heft-)Nummer: | 1 |
| DOI: | 10.1186/s13104-017-3032-8 |
| URL / URN: | https://doi.org/10.1186/s13104-017-3032-8 |
| Herkunft: | Zweitveröffentlichung aus gefördertem Golden Open Access |
| Kurzbeschreibung (Abstract): | Objective: Adhesion of cells to the extracellular matrix is facilitated by integrin receptors. We recently found that a nanoscale organization of plasma membrane located integrins containing the β1 subunit is responsible for an enhanced radio-resistance in 3D cultured cells over cells grown in 2D. While ionizing radiation is known to have broad effects on the lipid composition of the plasma membrane and their organization in lipid-rafts, it is not clear whether the effects of ionizing radiation on the nanoscale clustering of integrins is lipid-raft dependent. Results: Using single molecule microscopy we can show that β1 integrins colocalize with cholesterol in lipid-rafts. Ionizing radiation, as an extrinsic stressor, causes the separation of β1 integrins from cholesterol lipid raft suggesting that the effects of ionizing radiation on the clustering of β1 integrins are lipid-raft independent. |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-70428 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Membrane Dynamics |
| Hinterlegungsdatum: | 17 Dez 2017 20:55 |
| Letzte Änderung: | 03 Jun 2018 21:29 |
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- Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions. (deposited 17 Dez 2017 20:55) [Gegenwärtig angezeigt]
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