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The Sulfur Oxygenase Reductase Activity Assay: Catalyzing a Reaction with Elemental Sulfur as Substrate at High Temperatures

Rühl, Patrick ; Kletzin, Arnulf :
The Sulfur Oxygenase Reductase Activity Assay: Catalyzing a Reaction with Elemental Sulfur as Substrate at High Temperatures.
[Online-Edition: http://www.bio-protocol.org/e2403]
In: bio-protocol, 7 (14) ISSN 2331-8325
[Artikel], (2017)

Offizielle URL: http://www.bio-protocol.org/e2403
Typ des Eintrags: Artikel
Erschienen: 2017
Autor(en): Rühl, Patrick ; Kletzin, Arnulf
Titel: The Sulfur Oxygenase Reductase Activity Assay: Catalyzing a Reaction with Elemental Sulfur as Substrate at High Temperatures
Sprache: Englisch
Titel der Zeitschrift, Zeitung oder Schriftenreihe: bio-protocol
Band: 7
(Heft-)Nummer: 14
Verlag: bio-protocol
Freie Schlagworte: Sulfur oxygenase reductase, Elemental sulfur, Thermozyme, Sulfur disproportionation, Thiosulfate, Sulfite, Hydrogen sulfide
Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Sulfur Biochemistry and Microbial Bioenergetics
Hinterlegungsdatum: 17 Aug 2017 14:28
Offizielle URL: http://www.bio-protocol.org/e2403
Alternatives oder übersetztes Abstract:
AbstractSprache
The sulfur oxygenase reductase (SOR) reaction is a dioxygen-dependent disproportionation of elemental sulfur (S0), catalyzed at optimal temperatures between 65 °C and 85 °C. Thiosulfate and sulfite are formed as oxidized products as well hydrogen sulfide as reduced product. External co-factors are not required. Usually, the SOR assay is performed in a milliliter scale in S0-containing Tris-buffer at high temperatures followed by colorimetric product quantification. In order to make the SOR assay more sensitive and better reproducible, several modifications were implemented compared to the original SOR assay (Kletzin, 1989). Here we present the modified SOR assay and the following quantification of the reaction products.Englisch
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