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The Sulfur Oxygenase Reductase Activity Assay: Catalyzing a Reaction with Elemental Sulfur as Substrate at High Temperatures

Rühl, Patrick and Kletzin, Arnulf (2017):
The Sulfur Oxygenase Reductase Activity Assay: Catalyzing a Reaction with Elemental Sulfur as Substrate at High Temperatures.
In: bio-protocol, bio-protocol, 7, (14), ISSN 2331-8325,
DOI: 10.21769/BioProtoc.2403,
[Online-Edition: http://www.bio-protocol.org/e2403],
[Article]

Item Type: Article
Erschienen: 2017
Creators: Rühl, Patrick and Kletzin, Arnulf
Title: The Sulfur Oxygenase Reductase Activity Assay: Catalyzing a Reaction with Elemental Sulfur as Substrate at High Temperatures
Language: English
Journal or Publication Title: bio-protocol
Volume: 7
Number: 14
Publisher: bio-protocol
Uncontrolled Keywords: Sulfur oxygenase reductase, Elemental sulfur, Thermozyme, Sulfur disproportionation, Thiosulfate, Sulfite, Hydrogen sulfide
Divisions: 10 Department of Biology
10 Department of Biology > Sulfur Biochemistry and Microbial Bioenergetics
Date Deposited: 17 Aug 2017 14:28
DOI: 10.21769/BioProtoc.2403
Official URL: http://www.bio-protocol.org/e2403
Alternative Abstract:
Alternative abstract Language
The sulfur oxygenase reductase (SOR) reaction is a dioxygen-dependent disproportionation of elemental sulfur (S0), catalyzed at optimal temperatures between 65 °C and 85 °C. Thiosulfate and sulfite are formed as oxidized products as well hydrogen sulfide as reduced product. External co-factors are not required. Usually, the SOR assay is performed in a milliliter scale in S0-containing Tris-buffer at high temperatures followed by colorimetric product quantification. In order to make the SOR assay more sensitive and better reproducible, several modifications were implemented compared to the original SOR assay (Kletzin, 1989). Here we present the modified SOR assay and the following quantification of the reaction products.English
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