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Pausing guides RNA folding to populate transiently stable RNA structures for riboswitch-based transcription regulation.

Steinert, Hannah and Sochor, Florian and Wacker, Anna and Buck, Janina and Helmling, Christina and Hiller, Fabian and Keyhani, Sara and Noeske, Jonas and Grimm, Steffen Kaspar and Rudolph, Martin M. and Keller, Heiko and Mooney, Rachel Anne and Landick, Robert and Suess, Beatrix and Fürtig, Boris and Wöhnert, Jens and Schwalbe, Harald (2017):
Pausing guides RNA folding to populate transiently stable RNA structures for riboswitch-based transcription regulation.
In: eLife, pp. e21297, 6, ISSN 2050-084X, [Article]

Abstract

In bacteria, the regulation of gene expression by cis-acting transcriptional riboswitches located in the 5'-untranslated regions of messenger RNA requires the temporal synchronization of RNA synthesis and ligand binding-dependent conformational refolding. Ligand binding to the aptamer domain of the riboswitch induces premature termination of the mRNA synthesis of ligand-associated genes due to the coupled formation of 3'-structural elements acting as terminators. To date, there has been no high resolution structural description of the concerted process of synthesis and ligand-induced restructuring of the regulatory RNA element. Here, we show that for the guanine-sensing xpt-pbuX riboswitch from Bacillus subtilis, the conformation of the full-length transcripts is static: it exclusively populates the functional off-state but cannot switch to the on-state, regardless of the presence or absence of ligand. We show that only the combined matching of transcription rates and ligand binding enables transcription intermediates to undergo ligand-dependent conformational refolding.

Item Type: Article
Erschienen: 2017
Creators: Steinert, Hannah and Sochor, Florian and Wacker, Anna and Buck, Janina and Helmling, Christina and Hiller, Fabian and Keyhani, Sara and Noeske, Jonas and Grimm, Steffen Kaspar and Rudolph, Martin M. and Keller, Heiko and Mooney, Rachel Anne and Landick, Robert and Suess, Beatrix and Fürtig, Boris and Wöhnert, Jens and Schwalbe, Harald
Title: Pausing guides RNA folding to populate transiently stable RNA structures for riboswitch-based transcription regulation.
Language: English
Abstract:

In bacteria, the regulation of gene expression by cis-acting transcriptional riboswitches located in the 5'-untranslated regions of messenger RNA requires the temporal synchronization of RNA synthesis and ligand binding-dependent conformational refolding. Ligand binding to the aptamer domain of the riboswitch induces premature termination of the mRNA synthesis of ligand-associated genes due to the coupled formation of 3'-structural elements acting as terminators. To date, there has been no high resolution structural description of the concerted process of synthesis and ligand-induced restructuring of the regulatory RNA element. Here, we show that for the guanine-sensing xpt-pbuX riboswitch from Bacillus subtilis, the conformation of the full-length transcripts is static: it exclusively populates the functional off-state but cannot switch to the on-state, regardless of the presence or absence of ligand. We show that only the combined matching of transcription rates and ligand binding enables transcription intermediates to undergo ligand-dependent conformational refolding.

Journal or Publication Title: eLife
Volume: 6
Divisions: 10 Department of Biology
10 Department of Biology > Synthetic Genetic Circuits
Date Deposited: 30 May 2017 07:18
Identification Number: pmid:28541183
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