Ohuchi, Shoji ; Suess, Beatrix (2017)
An inhibitory RNA aptamer against the lambda cI repressor shows transcriptional activator activity in vivo.
In: FEBS letters, 591 (10)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
An RNA aptamer is one of the promising components for constructing artificial genetic circuits. In this study, we developed a transcriptional activator based on an RNA aptamer against one of the most frequently applied repressor proteins, lambda phage cI. In vitro selection (SELEX), followed by in vivo screening identified an RNA aptamer with the intended transcriptional activator activity from an RNA pool containing a 40-nucleotide long random region. Quantitative analysis showed 35-fold elevation of reporter expression upon aptamer expression. These results suggest that the diversity of artificial transcriptional activators can be extended by employing RNA aptamers against repressor proteins to broaden the tools available for constructing genetic circuits. This article is protected by copyright. All rights reserved.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2017 |
| Autor(en): | Ohuchi, Shoji ; Suess, Beatrix |
| Art des Eintrags: | Bibliographie |
| Titel: | An inhibitory RNA aptamer against the lambda cI repressor shows transcriptional activator activity in vivo. |
| Sprache: | Englisch |
| Publikationsjahr: | Mai 2017 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | FEBS letters |
| Jahrgang/Volume einer Zeitschrift: | 591 |
| (Heft-)Nummer: | 10 |
| Kurzbeschreibung (Abstract): | An RNA aptamer is one of the promising components for constructing artificial genetic circuits. In this study, we developed a transcriptional activator based on an RNA aptamer against one of the most frequently applied repressor proteins, lambda phage cI. In vitro selection (SELEX), followed by in vivo screening identified an RNA aptamer with the intended transcriptional activator activity from an RNA pool containing a 40-nucleotide long random region. Quantitative analysis showed 35-fold elevation of reporter expression upon aptamer expression. These results suggest that the diversity of artificial transcriptional activators can be extended by employing RNA aptamers against repressor proteins to broaden the tools available for constructing genetic circuits. This article is protected by copyright. All rights reserved. |
| ID-Nummer: | pmid:28407231 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Synthetic Genetic Circuits (2020 umbenannt in "Synthetic RNA biology") |
| Hinterlegungsdatum: | 19 Apr 2017 12:20 |
| Letzte Änderung: | 18 Jul 2017 09:19 |
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