Degreif, Daniel ; Rond, Tristan de ; Bertl, Adam ; Keasling, Jay D. ; Budin, Itay (2017)
Lipid engineering reveals regulatory roles for membrane fluidity in yeast flocculation and oxygen-limited growth.
In: Metabolic engineering, 41
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Cells modulate lipid metabolism in order to maintain membrane homeostasis. Here we use a metabolic engineering approach to manipulate the stoichiometry of fatty acid unsaturation, a regulator of cell membrane fluidity, in Saccharomyces cerevisiae. Unexpectedly, reduced lipid unsaturation triggered cell-cell adhesion (flocculation), a phenomenon characteristic of industrial yeast but uncommon in laboratory strains. We find that ER lipid saturation sensors induce expression of FLO1 - encoding a cell wall polysaccharide binding protein - independently of its canonical regulator. In wild-type cells, Flo1p-dependent flocculation occurs under oxygen-limited growth, which reduces unsaturated lipid synthesis and thus serves as the environmental trigger for flocculation. Transcriptional analysis shows that FLO1 is one of the most highly induced genes in response to changes in lipid unsaturation, and that the set of membrane fluidity-sensitive genes is globally activated as part of the cell's long-term response to hypoxia during fermentation. Our results show how the lipid homeostasis machinery of budding yeast is adapted to carry out a broad response to an environmental stimulus important in biotechnology.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2017 |
| Autor(en): | Degreif, Daniel ; Rond, Tristan de ; Bertl, Adam ; Keasling, Jay D. ; Budin, Itay |
| Art des Eintrags: | Bibliographie |
| Titel: | Lipid engineering reveals regulatory roles for membrane fluidity in yeast flocculation and oxygen-limited growth |
| Sprache: | Englisch |
| Publikationsjahr: | 18 März 2017 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Metabolic engineering |
| Jahrgang/Volume einer Zeitschrift: | 41 |
| Kurzbeschreibung (Abstract): | Cells modulate lipid metabolism in order to maintain membrane homeostasis. Here we use a metabolic engineering approach to manipulate the stoichiometry of fatty acid unsaturation, a regulator of cell membrane fluidity, in Saccharomyces cerevisiae. Unexpectedly, reduced lipid unsaturation triggered cell-cell adhesion (flocculation), a phenomenon characteristic of industrial yeast but uncommon in laboratory strains. We find that ER lipid saturation sensors induce expression of FLO1 - encoding a cell wall polysaccharide binding protein - independently of its canonical regulator. In wild-type cells, Flo1p-dependent flocculation occurs under oxygen-limited growth, which reduces unsaturated lipid synthesis and thus serves as the environmental trigger for flocculation. Transcriptional analysis shows that FLO1 is one of the most highly induced genes in response to changes in lipid unsaturation, and that the set of membrane fluidity-sensitive genes is globally activated as part of the cell's long-term response to hypoxia during fermentation. Our results show how the lipid homeostasis machinery of budding yeast is adapted to carry out a broad response to an environmental stimulus important in biotechnology. |
| ID-Nummer: | pmid:28323063 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Yeast Membrane Biology |
| Hinterlegungsdatum: | 10 Apr 2017 10:46 |
| Letzte Änderung: | 20 Aug 2021 11:45 |
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