Siddique, Asma ; Meckel, Tobias ; Stark, Robert W. ; Narayan, Suman (2017)
Improved cell adhesion under shear stress in PDMS microfluidic devices.
In: Colloids and Surfaces B-Biointerfaces, 150
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Microfluidic systems based on polydimethylsiloxane (PDMS) provide a versatile platform to study the mechanoresponse of cells in vitro. Under a shear flow, however, the stability of cells that were grown on physically adsorbed proteins is short lived, which limits long-term cell studies. To address this issue, we used (3-Aminopropyl)triethoxysilane (APTES) as a linker between PDMS and collagen. In micro-channels that were modified with APTES-anchored collagen, fibroblast cells demonstrated higher stability and better proliferation as compared to collagen that was physically adsorbed onto PDMS after oxygen plasma treatment. To assess the stability of the cellular adhesion, cells were forced in a shear flow until detachment. In devices with APTES-anchored collagen, cells showed better adhesion and proliferation at shear stresses between 11.6 and 93 dyn/cm2 as compared to devices with the adsorbed collagen coating where the first cellular detachment occurred already at a shear stress of 23 dyn/cm2. The APTES-attached collagen coating also contributed to an improved long-term cellular growth (observed for 48 h) at different shear stress levels (10–300 dyn/cm2). Attachment of collagen with the help of APTES thus is a very promising technique not only to modify the glass but also to modify the PDMS surfaces of microfluidic devices for mechanotransduction experiments.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2017 |
| Autor(en): | Siddique, Asma ; Meckel, Tobias ; Stark, Robert W. ; Narayan, Suman |
| Art des Eintrags: | Bibliographie |
| Titel: | Improved cell adhesion under shear stress in PDMS microfluidic devices |
| Sprache: | Englisch |
| Publikationsjahr: | 1 Februar 2017 |
| Verlag: | Elsevier Science Publishing |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Colloids and Surfaces B-Biointerfaces |
| Jahrgang/Volume einer Zeitschrift: | 150 |
| URL / URN: | http://dx.doi.org/10.1016/j.colsurfb.2016.11.011 |
| Kurzbeschreibung (Abstract): | Microfluidic systems based on polydimethylsiloxane (PDMS) provide a versatile platform to study the mechanoresponse of cells in vitro. Under a shear flow, however, the stability of cells that were grown on physically adsorbed proteins is short lived, which limits long-term cell studies. To address this issue, we used (3-Aminopropyl)triethoxysilane (APTES) as a linker between PDMS and collagen. In micro-channels that were modified with APTES-anchored collagen, fibroblast cells demonstrated higher stability and better proliferation as compared to collagen that was physically adsorbed onto PDMS after oxygen plasma treatment. To assess the stability of the cellular adhesion, cells were forced in a shear flow until detachment. In devices with APTES-anchored collagen, cells showed better adhesion and proliferation at shear stresses between 11.6 and 93 dyn/cm2 as compared to devices with the adsorbed collagen coating where the first cellular detachment occurred already at a shear stress of 23 dyn/cm2. The APTES-attached collagen coating also contributed to an improved long-term cellular growth (observed for 48 h) at different shear stress levels (10–300 dyn/cm2). Attachment of collagen with the help of APTES thus is a very promising technique not only to modify the glass but also to modify the PDMS surfaces of microfluidic devices for mechanotransduction experiments. |
| Freie Schlagworte: | Microfluidics, Cell adhesion, Shear stress, APTES, PDMS, Surface modification |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Membrane Dynamics 11 Fachbereich Material- und Geowissenschaften 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft > Fachgebiet Physics of Surfaces DFG-Sonderforschungsbereiche (inkl. Transregio) DFG-Sonderforschungsbereiche (inkl. Transregio) > Sonderforschungsbereiche DFG-Sonderforschungsbereiche (inkl. Transregio) > Sonderforschungsbereiche > SFB 1194: Wechselseitige Beeinflussung von Transport- und Benetzungsvorgängen DFG-Sonderforschungsbereiche (inkl. Transregio) > Sonderforschungsbereiche > SFB 1194: Wechselseitige Beeinflussung von Transport- und Benetzungsvorgängen > Projektbereich A: Generische Experimente DFG-Sonderforschungsbereiche (inkl. Transregio) > Sonderforschungsbereiche > SFB 1194: Wechselseitige Beeinflussung von Transport- und Benetzungsvorgängen > Projektbereich A: Generische Experimente > A07: Raman-spektroskopische Untersuchung des Massetransports und des Konzentrationsgefälles in Gemischen Profilbereiche Profilbereiche > Thermo-Fluids & Interfaces |
| Hinterlegungsdatum: | 03 Apr 2017 10:12 |
| Letzte Änderung: | 19 Sep 2019 05:59 |
| PPN: | |
| Sponsoren: | A. S. acknowledges Deutscher Akademischer Austauschdienst (DAAD) and Higher Education Commission (HEC) Pakistan for the doctoral scholarship in Germany. |
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