Cosentino, Cristian ; Alberio, Laura ; Thiel, Gerhard ; Moroni, Anna (2017)
Yeast-Based Screening System for the Selection of Functional Light-Driven K(+) Channels.
In: Methods in molecular biology (Clifton, N.J.), 1596
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Ion channels control the electrical properties of cells by opening and closing (gating) in response to a wide palette of environmental and physiological stimuli. Endowing ion channels with the possibility to be gated by remotely applied stimuli, such as light, provides a tool for in vivo control of cellular functions in behaving animals. We have engineered a synthetic light-gated potassium (K(+)) channel by connecting an exogenous plant photoreceptor LOV2 domain to the K(+) channel pore Kcv. Here, we describe the experimental strategy that we have used to evolve the properties of the channel toward full control of light on pore gating. Our method combines rational and random mutagenesis of the channel followed by a yeast-based screening system for light-activated K(+) conductance.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2017 |
| Autor(en): | Cosentino, Cristian ; Alberio, Laura ; Thiel, Gerhard ; Moroni, Anna |
| Art des Eintrags: | Bibliographie |
| Titel: | Yeast-Based Screening System for the Selection of Functional Light-Driven K(+) Channels. |
| Sprache: | Englisch |
| Publikationsjahr: | 2017 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Methods in molecular biology (Clifton, N.J.) |
| Jahrgang/Volume einer Zeitschrift: | 1596 |
| Kurzbeschreibung (Abstract): | Ion channels control the electrical properties of cells by opening and closing (gating) in response to a wide palette of environmental and physiological stimuli. Endowing ion channels with the possibility to be gated by remotely applied stimuli, such as light, provides a tool for in vivo control of cellular functions in behaving animals. We have engineered a synthetic light-gated potassium (K(+)) channel by connecting an exogenous plant photoreceptor LOV2 domain to the K(+) channel pore Kcv. Here, we describe the experimental strategy that we have used to evolve the properties of the channel toward full control of light on pore gating. Our method combines rational and random mutagenesis of the channel followed by a yeast-based screening system for light-activated K(+) conductance. |
| ID-Nummer: | pmid:28293893 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) |
| Hinterlegungsdatum: | 23 Mär 2017 07:59 |
| Letzte Änderung: | 23 Mär 2017 07:59 |
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