Guo, Zhong ; Murphy, Lindy ; Stein, Viktor ; Johnston, Wayne A. ; Alcala-Perez, Siro ; Alexandrov, Kirill (2016)
Engineered PQQ-Glucose Dehydrogenase as a Universal Biosensor Platform.
In: Journal of the American Chemical Society, 138 (32)
doi: 10.1021/jacs.6b06342
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Biosensors with direct electron output hold promise for nearly seamless integration with portable electronic devices. However, so far, they have been based on naturally occurring enzymes that significantly limit the spectrum of detectable analytes. Here, we present a novel biosensor architecture based on analyte-driven intermolecular recombination and activity reconstitution of a re-engineered component of glucometers: PQQ-glucose dehydrogenase. We demonstrate that this sensor architecture can be rapidly adopted for the detection of immunosuppressant drugs, α-amylase protein, or protease activity of thrombin and Factor Xa. The biosensors could be stored in dried form without appreciable loss of activity. We further show that ligand-induced activity of the developed biosensors could be directly monitored by chronoamperometry, enabling construction of disposable sensory electrodes. We expect that this architecture could be expanded to the detection of other biochemical activities, post-translational modifications, nucleic acids, and inorganic molecules.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2016 |
| Autor(en): | Guo, Zhong ; Murphy, Lindy ; Stein, Viktor ; Johnston, Wayne A. ; Alcala-Perez, Siro ; Alexandrov, Kirill |
| Art des Eintrags: | Bibliographie |
| Titel: | Engineered PQQ-Glucose Dehydrogenase as a Universal Biosensor Platform. |
| Sprache: | Englisch |
| Publikationsjahr: | 2016 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of the American Chemical Society |
| Jahrgang/Volume einer Zeitschrift: | 138 |
| (Heft-)Nummer: | 32 |
| DOI: | 10.1021/jacs.6b06342 |
| Kurzbeschreibung (Abstract): | Biosensors with direct electron output hold promise for nearly seamless integration with portable electronic devices. However, so far, they have been based on naturally occurring enzymes that significantly limit the spectrum of detectable analytes. Here, we present a novel biosensor architecture based on analyte-driven intermolecular recombination and activity reconstitution of a re-engineered component of glucometers: PQQ-glucose dehydrogenase. We demonstrate that this sensor architecture can be rapidly adopted for the detection of immunosuppressant drugs, α-amylase protein, or protease activity of thrombin and Factor Xa. The biosensors could be stored in dried form without appreciable loss of activity. We further show that ligand-induced activity of the developed biosensors could be directly monitored by chronoamperometry, enabling construction of disposable sensory electrodes. We expect that this architecture could be expanded to the detection of other biochemical activities, post-translational modifications, nucleic acids, and inorganic molecules. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Protein Engineering of Ion Conducting Nanopores 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 14 Nov 2016 10:52 |
| Letzte Änderung: | 22 Nov 2016 11:16 |
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