Zhang, Ying ; Schäffer, Thea ; Wölfle, Tina ; Fitzke, Edith ; Thiel, Gerhard ; Rospert, Sabine (2016)
Cotranslational intersection between the SRP and GET targeting pathways to the ER of Saccharomyces cerevisiae.
In: Molecular and cellular biology, 36 (18)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Targeting of transmembrane proteins to the endoplasmic reticulum proceeds via either the SRP or the GET pathway, consisting of Get1-5 and Sgt2. While SRP cotranslationally targets membrane proteins containing one or multiple transmembrane domains, the GET pathway posttranslationally targets proteins containing a single, C-terminal transmembrane domain termed tail-anchor. Here we dissect the role of the SRP and GET pathway in the sorting of homologous, two-membrane spanning K(+) channel proteins termed Kcv, Kesv, and Kesv-VV. We show that Kcv was targeted to the ER cotranslationally via its N-terminal transmembrane domain, while Kesv-VV was targeted posttranslationally via its C-terminal transmembrane domain, which recruited Get4-5/Sgt2 and Get3. Unexpectedly, nascent Kcv did not only recruit SRP, but also the Get4-5 module of the GET pathway to ribosomes. Ribosome-binding of Get4-5 was independent of Sgt2 and was strongly outcompeted by SRP. The combined data indicate a previously unrecognized cotranslational interplay between the SRP and GET pathways.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2016 |
| Autor(en): | Zhang, Ying ; Schäffer, Thea ; Wölfle, Tina ; Fitzke, Edith ; Thiel, Gerhard ; Rospert, Sabine |
| Art des Eintrags: | Bibliographie |
| Titel: | Cotranslational intersection between the SRP and GET targeting pathways to the ER of Saccharomyces cerevisiae. |
| Sprache: | Englisch |
| Publikationsjahr: | 2016 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Molecular and cellular biology |
| Jahrgang/Volume einer Zeitschrift: | 36 |
| (Heft-)Nummer: | 18 |
| Kurzbeschreibung (Abstract): | Targeting of transmembrane proteins to the endoplasmic reticulum proceeds via either the SRP or the GET pathway, consisting of Get1-5 and Sgt2. While SRP cotranslationally targets membrane proteins containing one or multiple transmembrane domains, the GET pathway posttranslationally targets proteins containing a single, C-terminal transmembrane domain termed tail-anchor. Here we dissect the role of the SRP and GET pathway in the sorting of homologous, two-membrane spanning K(+) channel proteins termed Kcv, Kesv, and Kesv-VV. We show that Kcv was targeted to the ER cotranslationally via its N-terminal transmembrane domain, while Kesv-VV was targeted posttranslationally via its C-terminal transmembrane domain, which recruited Get4-5/Sgt2 and Get3. Unexpectedly, nascent Kcv did not only recruit SRP, but also the Get4-5 module of the GET pathway to ribosomes. Ribosome-binding of Get4-5 was independent of Sgt2 and was strongly outcompeted by SRP. The combined data indicate a previously unrecognized cotranslational interplay between the SRP and GET pathways. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) |
| Hinterlegungsdatum: | 05 Jul 2016 08:40 |
| Letzte Änderung: | 04 Okt 2016 07:39 |
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