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Label-Free Pyrophosphate Recognition with Functionalized Asymmetric Nanopores

Ali, Mubarak ; Ahmed, Ishtiaq ; Ramirez, Patricio ; Nasir, Saima ; Niemeyer, Christof M. ; Mafe, Salvador ; Ensinger, Wolfgang :
Label-Free Pyrophosphate Recognition with Functionalized Asymmetric Nanopores.
[Online-Edition: http://dx.doi.org/10.1002/smll.201600160]
In: Small, 12 (15) pp. 2014-2021. ISSN 16136810
[Artikel], (2016)

Offizielle URL: http://dx.doi.org/10.1002/smll.201600160

Kurzbeschreibung (Abstract)

The label-free detection of pyrophosphate (PPi) anions with a nanofluidic sensing device based on asymmetric nanopores is demonstrated. The pore surface is functionalized with zinc complexes based on two di(2-picolyl)amine [bis(DPA)] moieties using carbodiimide coupling chemistry. The complexation of zinc (Zn2+) ion is achieved by exposing the modified pore to a solution of zinc chloride to form bis(Zn2+-DPA) complexes. The chemical functionalization is demonstrated by recording the changes in the observed current-voltage (I-V) curves before and after pore modification. The bis(Zn2+-DPA) complexes on the pore walls serve as recognition sites for pyrophosphate anion. The experimental results show that the proposed nanofluidic sensor has the ability to sense picomolar concentrations of PPi anion in the surrounding environment. On the contrary, it does not respond to other phosphate anions, including monohydrogen phosphate, dihydrogen phosphate, adenosine monophosphate, adenosine diphosphate, and adenosine triphosphate. The experimental results are described theoretically by using a model based on the Poisson-Nernst-Planck equations.

Typ des Eintrags: Artikel
Erschienen: 2016
Autor(en): Ali, Mubarak ; Ahmed, Ishtiaq ; Ramirez, Patricio ; Nasir, Saima ; Niemeyer, Christof M. ; Mafe, Salvador ; Ensinger, Wolfgang
Titel: Label-Free Pyrophosphate Recognition with Functionalized Asymmetric Nanopores
Sprache: Englisch
Kurzbeschreibung (Abstract):

The label-free detection of pyrophosphate (PPi) anions with a nanofluidic sensing device based on asymmetric nanopores is demonstrated. The pore surface is functionalized with zinc complexes based on two di(2-picolyl)amine [bis(DPA)] moieties using carbodiimide coupling chemistry. The complexation of zinc (Zn2+) ion is achieved by exposing the modified pore to a solution of zinc chloride to form bis(Zn2+-DPA) complexes. The chemical functionalization is demonstrated by recording the changes in the observed current-voltage (I-V) curves before and after pore modification. The bis(Zn2+-DPA) complexes on the pore walls serve as recognition sites for pyrophosphate anion. The experimental results show that the proposed nanofluidic sensor has the ability to sense picomolar concentrations of PPi anion in the surrounding environment. On the contrary, it does not respond to other phosphate anions, including monohydrogen phosphate, dihydrogen phosphate, adenosine monophosphate, adenosine diphosphate, and adenosine triphosphate. The experimental results are described theoretically by using a model based on the Poisson-Nernst-Planck equations.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: Small
Band: 12
(Heft-)Nummer: 15
Verlag: WILEY-V C H VERLAG GMBH, WEINHEIM, GERMANY
Freie Schlagworte: current rectification, functionalization, sensors, pyrophosphate, track-etching, Zn(II)-dipicolylamine complexes
Fachbereich(e)/-gebiet(e): 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft > Fachgebiet Materialanalytik
11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft
11 Fachbereich Material- und Geowissenschaften
Hinterlegungsdatum: 06 Jun 2016 11:55
Offizielle URL: http://dx.doi.org/10.1002/smll.201600160
ID-Nummer: 10.1002/smll.201600160
Sponsoren: M.A., S.N., and W.E. acknowledge the funding from the Hessen State Ministry of Higher Education, Research and the Arts, Germany, under the LOEWE project iNAPO., P.R. and S.M. acknowledge financial support by the Generalitat Valenciana (Program of Excellence Prometeo/GV/0069), the Spanish Ministry of Economic Affairs and Competitiveness (MAT2015-65011-P), and FEDER.I. A. and C.M.N. acknowledge financial support th, I. A. and C.M.N. acknowledge financial support through the Helmholtz programme BioInterfaces in Technology and Medicine.
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