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Label-Free Pyrophosphate Recognition with Functionalized Asymmetric Nanopores

Ali, Mubarak and Ahmed, Ishtiaq and Ramirez, Patricio and Nasir, Saima and Niemeyer, Christof M. and Mafe, Salvador and Ensinger, Wolfgang :
Label-Free Pyrophosphate Recognition with Functionalized Asymmetric Nanopores.
[Online-Edition: http://dx.doi.org/10.1002/smll.201600160]
In: Small, 12 (15) pp. 2014-2021. ISSN 16136810
[Article] , (2016)

Official URL: http://dx.doi.org/10.1002/smll.201600160

Abstract

The label-free detection of pyrophosphate (PPi) anions with a nanofluidic sensing device based on asymmetric nanopores is demonstrated. The pore surface is functionalized with zinc complexes based on two di(2-picolyl)amine [bis(DPA)] moieties using carbodiimide coupling chemistry. The complexation of zinc (Zn2+) ion is achieved by exposing the modified pore to a solution of zinc chloride to form bis(Zn2+-DPA) complexes. The chemical functionalization is demonstrated by recording the changes in the observed current-voltage (I-V) curves before and after pore modification. The bis(Zn2+-DPA) complexes on the pore walls serve as recognition sites for pyrophosphate anion. The experimental results show that the proposed nanofluidic sensor has the ability to sense picomolar concentrations of PPi anion in the surrounding environment. On the contrary, it does not respond to other phosphate anions, including monohydrogen phosphate, dihydrogen phosphate, adenosine monophosphate, adenosine diphosphate, and adenosine triphosphate. The experimental results are described theoretically by using a model based on the Poisson-Nernst-Planck equations.

Item Type: Article
Erschienen: 2016
Creators: Ali, Mubarak and Ahmed, Ishtiaq and Ramirez, Patricio and Nasir, Saima and Niemeyer, Christof M. and Mafe, Salvador and Ensinger, Wolfgang
Title: Label-Free Pyrophosphate Recognition with Functionalized Asymmetric Nanopores
Language: English
Abstract:

The label-free detection of pyrophosphate (PPi) anions with a nanofluidic sensing device based on asymmetric nanopores is demonstrated. The pore surface is functionalized with zinc complexes based on two di(2-picolyl)amine [bis(DPA)] moieties using carbodiimide coupling chemistry. The complexation of zinc (Zn2+) ion is achieved by exposing the modified pore to a solution of zinc chloride to form bis(Zn2+-DPA) complexes. The chemical functionalization is demonstrated by recording the changes in the observed current-voltage (I-V) curves before and after pore modification. The bis(Zn2+-DPA) complexes on the pore walls serve as recognition sites for pyrophosphate anion. The experimental results show that the proposed nanofluidic sensor has the ability to sense picomolar concentrations of PPi anion in the surrounding environment. On the contrary, it does not respond to other phosphate anions, including monohydrogen phosphate, dihydrogen phosphate, adenosine monophosphate, adenosine diphosphate, and adenosine triphosphate. The experimental results are described theoretically by using a model based on the Poisson-Nernst-Planck equations.

Journal or Publication Title: Small
Volume: 12
Number: 15
Publisher: WILEY-V C H VERLAG GMBH, WEINHEIM, GERMANY
Uncontrolled Keywords: current rectification, functionalization, sensors, pyrophosphate, track-etching, Zn(II)-dipicolylamine complexes
Divisions: 11 Department of Materials and Earth Sciences > Material Science > Material Analytics
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences
Date Deposited: 06 Jun 2016 11:55
Official URL: http://dx.doi.org/10.1002/smll.201600160
Identification Number: doi:10.1002/smll.201600160
Funders: M.A., S.N., and W.E. acknowledge the funding from the Hessen State Ministry of Higher Education, Research and the Arts, Germany, under the LOEWE project iNAPO., P.R. and S.M. acknowledge financial support by the Generalitat Valenciana (Program of Excellence Prometeo/GV/0069), the Spanish Ministry of Economic Affairs and Competitiveness (MAT2015-65011-P), and FEDER.I. A. and C.M.N. acknowledge financial support th, I. A. and C.M.N. acknowledge financial support through the Helmholtz programme BioInterfaces in Technology and Medicine.
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