Groher, Florian ; Suess, Beatrix (2016)
In vitro selection of antibiotic-binding aptamers.
In: Methods (San Diego, Calif.), 106
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Despite its wide applicability the selection of small molecule-binding RNA aptamers with high affinity binding and specificity is still challenging. We will present here a protocol which allows the in vitro selection of antibiotic-binding aptamers which turned out to be important building blocks for the design process of synthetic riboswitches. The presented methods will be compared with alternative in vitro selection protocols. A detailed note section will point out useful tips and pitfalls.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2016 |
| Autor(en): | Groher, Florian ; Suess, Beatrix |
| Art des Eintrags: | Bibliographie |
| Titel: | In vitro selection of antibiotic-binding aptamers. |
| Sprache: | Englisch |
| Publikationsjahr: | 2016 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Methods (San Diego, Calif.) |
| Jahrgang/Volume einer Zeitschrift: | 106 |
| Kurzbeschreibung (Abstract): | Despite its wide applicability the selection of small molecule-binding RNA aptamers with high affinity binding and specificity is still challenging. We will present here a protocol which allows the in vitro selection of antibiotic-binding aptamers which turned out to be important building blocks for the design process of synthetic riboswitches. The presented methods will be compared with alternative in vitro selection protocols. A detailed note section will point out useful tips and pitfalls. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Synthetic Genetic Circuits (2020 umbenannt in "Synthetic RNA biology") |
| Hinterlegungsdatum: | 31 Mai 2016 06:46 |
| Letzte Änderung: | 20 Sep 2016 05:47 |
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